Purified anti-mouse IL-1α Antibody

Pricing & Availability
Clone
ALF-161 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)
Isotype
Armenian Hamster IgG
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Product Citations
publications
Mouse_IL-1alpha_020305
  • Mouse_IL-1alpha_020305
  • zALF-161_IF_072412
    Immortalized murine bone marrow-derived macrophages stimulated with LPS (100 ng/ml) for 24 h, fixed with 2% paraformaldehyde, permeabilized with 0.1% Triton X100, stained with IL-1α (clone ALF-161), followed by DyLight® 594 goat anti-hamster Ig (red). Cells also express GFP-LC3 (green). Image was visualized on an Olympus FV1000 confocal at 63x. Images provided by James Harris and Celia Peral de Castro, Trinity College.
Cat # Size Price Quantity Avail. Save
503202 500 µg 115 CHF
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The ALF-161 antibody reacts with precursor, secreted and membrane-associated forms of mouse IL-1α. The ALF-161 antibody can neutralize the bioactivity of natural or recombinant IL-1α.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli - expressed, recombinant mouse IL-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IF - Verified
WB, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1.0 - 4.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture: The purified ALF-161 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5034 antibody (Cat. No. 503401) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
ELISA Capture: The purified ALF-161 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated BL1a-89 antibody (Cat. Nos. 512503 & 512504) as the detection antibody and recombinant mouse IL-1a (Cat. No. 580309) as the standard.
Neutralization: Clone ALF-161 has been described to have IL-1a neutralizing properties based on the in vivo mice model3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended (Cat. No. 503207-503212).

Application References

(PubMed link indicates BioLegend citation)
  1. Fuhlbrigge R, et al. 1988. J. Immunol. 141:2643.
  2. Rogers HW, et al. 1992. P. Natl. Acad.Sci. USA 89:1011.
  3. Yazdi AS, et al. 2010. P. Natl. Acad. Sci. USA 107:19449. (Neut)
RRID
AB_315280 (BioLegend Cat. No. 503202)

Antigen Details

Structure
Cytokine; 17.4 kD (Mammalian)
Bioactivity
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
Cell Targets
B cells, T cells, monocytes, fibroblasts
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Regulation
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID
16175 View all products for this Gene ID
UniProt
View information about IL-1alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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