Purified anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
1_RA3-6B2_Pure_112911
C57BL/6 mouse slenocytes were stained with purified B220 (clone RA3-6B2) (filled histogram) or rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
  • 1_RA3-6B2_Pure_112911
    C57BL/6 mouse slenocytes were stained with purified B220 (clone RA3-6B2) (filled histogram) or rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
  • 2_RA3-6B2_PURE_CD45R_IHC-F_091018
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of purified anti-mouse/human CD45R/B220 (clone RA3-6B2) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 594 (red) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 488 (green) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • RA3-6B2_PURE_CD45R_IHC-F_102418
    Fresh, frozen mouse spleen was stained with purified CD45R/B220 clone Ra3-6B2 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCT A647 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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103201 50 µg 25,00 CHF
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103202 500 µg 75,00 CHF
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
IHC-P, IP, Activ - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  2. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  3. Alrefai H, et al. 2016. Nat Commun. 7:11724. PubMed
  4. Fujimura N, et al. 2016. J Vasc Surg. 64: 46-54. PubMed
  5. Dungubat E, et al. 2020. Nutrients. 12:00. PubMed
  6. Feng X, et al. 2020. DNA Repair (Amst). 86:102754. PubMed
  7. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
  8. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  9. Heib T, et al. 2021. Cell Reports. 35(6):109102. PubMed
  10. Nagashima K, et al. 2017. Biochem Biophys Res Commun. . 10.1016/j.bbrc.2017.09.004. PubMed
  11. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  12. Gröschel C, et al. 2017. Sci Rep.. 10.1038/s41598-017-16147-1. PubMed
  13. Quaranta V, et al. 2018. Cancer Res. 2018:5379085. PubMed
  14. Grohmann M et al. 2018. Cell. 175(5):1289-1306 . PubMed
  15. De Schepper S et al. 2018. Cell. 175(2):400-415 . PubMed
  16. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  17. Adamson SE, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1020. PubMed
  18. Baluk P, et al. 2018. Methods Mol Biol. 1846:161. PubMed
  19. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  20. Chung WY, et al. 2018. ALTEX. 36:29. PubMed
  21. Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed
  22. Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
  23. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  24. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  25. Wang HC, et al. 2017. J Immunol. 198:3149. PubMed
  26. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  27. Schneppenheim J, et al. 2017. J Immunol. 199:172. PubMed
  28. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  29. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  30. Chiu H, et al. 2017. Front Immunol. 0.852083333. PubMed
  31. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  32. Goel S, et al. 2017. Nature. 548:471. PubMed
  33. Wang W, et al. 2018. J Vasc Surg. 68:1538. PubMed
  34. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  35. Duncan CG, et al. 2018. G3 (Bethesda). 0.892361111. PubMed
  36. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  37. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  38. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  39. Fonseca JA, et al. 2018. Vaccine. 36:2799. PubMed
  40. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  41. Wan X, et al. 2018. Nature. 560:107. PubMed
  42. Ito Y, et al. 2018. J Exp Med. 215:2617. PubMed
  43. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  44. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  45. Piliponsky AM, et al. 2019. Nat Immunol. 0.922916667. PubMed
  46. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  47. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  48. Stebegg M, et al. 2019. Nat Commun. 2.113194444. PubMed
  49. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  50. Ng SR, et al. 2020. Proc Natl Acad Sci U S A. 117:513. PubMed
  51. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  52. Cole C, et al. 2018. Nucleic Acids Res. 46:e62. PubMed
  53. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
  54. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  55. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  56. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  57. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  58. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  59. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  60. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
  61. Umemoto T, et al. 2017. EMBO J. 36:2390. PubMed
  62. Luo W, et al. 2019. Nat Immunol. 20:736. PubMed
  63. Mainini F, et al. 2018. Nucleic Acid Ther. 28:225. PubMed
  64. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
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RRID
AB_312986 (BioLegend Cat. No. 103201)
AB_312987 (BioLegend Cat. No. 103202)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04.25.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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