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- Clone
- S18017D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FcRI
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b APC and purified anti-mouse CD64 (FcγRI) (clone S18017D) (left) or purified anti-rat IgG2a, κ isotype control (right), followed by goat anti-rat IgG PE. Data shown was gated on myeloid cells. -
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C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of Purified anti-mouse CD64 (FcγRI) Antibody (clone S18017D) overnight at 4°C. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 anti-rat IgG (clone Poly4054, red) for two hours at room temperature. The image was captured with a 10X objective.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 161002 | 100 µg | 118 CHF | ||||
CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse FcγRI/CD64 (Cat. No. 773806)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2890725 (BioLegend Cat. No. 161002)
Antigen Details
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
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Monocytes, macrophages, mast cells, dendritic cells
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG
- Antigen References
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1. Schyns J, et al. 2019. Nat. Commun. 3;10(1):3964
2. Langlet C, et al. 2012. J. Immunol. 188 (4) 1751-1760. - Gene ID
- 14129 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
Related FAQs
Other Formats
View All CD64 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD64 (FcγRI) Antibody | S18017D | FC,IHC-F |
| PE anti-mouse CD64 (FcγRI) | S18017D | FC |
| APC anti-mouse CD64 (FcγRI) | S18017D | FC |
| FITC anti-mouse CD64 (FcγRI) | S18017D | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD64 (FcγRI) Antibody
C57BL/6 mouse bone marrow cells were stained with anti-mouse... 
C57BL/6 mouse frozen liver section was fixed with 4% parafor... -
PE anti-mouse CD64 (FcγRI)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... 
Human peripheral blood monocytes were stained with anti-mous... 
Human peripheral blood cells were stained with anti-mouse/hu... -
APC anti-mouse CD64 (FcγRI)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
FITC anti-mouse CD64 (FcγRI)
C57BL/6 mouse bone marrow cells were stained with anti-mouse...
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