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Purified anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
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Product Citations
publications
RM4-5_Pure_030206
C57BL/6 mouse splenocytes stained with purified CD4 (clone RM4-5) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram).
  • RM4-5_Pure_030206
    C57BL/6 mouse splenocytes stained with purified CD4 (clone RM4-5) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram).
  • RM4-5_Pure_CD4_Antibody_2_100219
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse CD4 (clone RM4-5), and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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100505 50 µg 30 CHF
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100506 500 µg 84 CHF
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  2. Abdel-Gadir A, et al. 2019. Nat Med. 25:1164. PubMed
  3. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  4. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  5. Tersteegen A, et al. 2021. Infect Immun. 89:. PubMed
  6. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  7. Vitiello GA, et al. 2018. Clin Cancer Res. 24:972. PubMed
  8. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  9. Bignon A, et al. 2017. Front Immunol. 0.722222222. PubMed
  10. Kondo H, et al. 2020. J Biol Chem. 295:1658. PubMed
  11. Leborgne NGF, et al. 2020. Front Immunol. 11:562587. PubMed
  12. Wang C, et al. 2020. Oncoimmunology. 9:1809947. PubMed
  13. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  14. Goel S, et al. 2017. Nature. 548:471. PubMed
  15. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  16. Shi B, et al. 2018. J Immunol. 200:586. PubMed
  17. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  18. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
  19. Mayer JU, et al. 2020. Front Immunol. 11:592325. PubMed
  20. Son YM, et al. 2020. Eur J Immunol. 50:1067. PubMed
  21. Nguyen N, et al. 2022. iScience. 25:103679. PubMed
  22. Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed
  23. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  24. Tian D, et al. 2020. FASEB J. 34:3367. PubMed
  25. Muhammad F, et al. 2020. Front Immunol. 975:11. PubMed
  26. Do DC, et al. 2019. JCI Insight. 4:e126832. PubMed
  27. Stebegg M, et al. 2019. Nat Commun. 2.113194444. PubMed
  28. Kaisar MMM, et al. 2018. PLoS Biol. 16:e2005504. PubMed
  29. Delgobo M, et al. 2021. Front Immunol. 12:584538. PubMed
  30. Li W, et al. 2020. J Clin Invest. 130:6718. PubMed
  31. Hiramatsu-Asano S, et al. 2021. Front Immunol. 11:616141. PubMed
  32. Lynn MA, et al. 2021. Cell Reports. 36(8):109564. PubMed
  33. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  34. Guo Y, et al. 2008. Blood. 112:480. PubMed
  35. Schuhmann MK, et al. 2021. J Neuroinflammation. 18:46. PubMed
  36. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  37. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  38. Hua J, et al. 2018. Sci Rep. 5.235416667. PubMed
  39. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  40. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  41. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  42. Vukman KV, et al. 2020. J Extracell Vesicles. 10:e12023. PubMed
  43. Brown IK, et al. 2021. PLoS Pathog. 17:e1009602. PubMed
  44. Yshii L, et al. 2022. Nat Immunol. 23:878. PubMed
  45. Umemoto T, et al. 2017. EMBO J. 36:2390. PubMed
  46. Chen X, et al. 2022. Front Immunol. 13:828319. PubMed
  47. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  48. Dooley K, et al. 2021. Mol Ther. 29:1729. PubMed
  49. Park GY, et al. 2020. Immune Netw. e43:20. PubMed
  50. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
  51. Van De Velde LA, et al. 2017. J Biol Chem. 292:15:00. PubMed
  52. Chihara N, et al. 2018. Nature. 558:454. PubMed
  53. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  54. Sun Y, et al. 2020. J Immunol. 205:3358. PubMed
  55. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  56. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  57. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  58. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  59. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  60. Costa B, et al. 2021. Cancers (Basel). 13:00. PubMed
  61. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  62. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  63. Williams SK, et al. 2018. Sci Rep. 8:13628. PubMed
  64. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
  65. Pasciuto E, et al. 2020. Cell. 182:625. PubMed
  66. Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed
  67. Li J, et al. 2021. Front Immunol. 12:710406. PubMed
  68. Schuhmann MK, et al. 2020. Circ Res. 127:1023. PubMed
  69. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  70. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  71. Seo SU, et al. 2021. Front Immunol. 12:697162. PubMed
  72. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  73. Yi J, et al. 2019. Mol Cells. 42:228. PubMed
  74. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  75. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  76. Deng W et al. 2019. Cell reports. 27(6):1755-1768 . PubMed
  77. Takeuchi Y, et al. 2021. Sci Rep. 11:18679. PubMed
  78. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  79. Lee YJ, et al. 2018. Front Microbiol. 9:83. PubMed
  80. Heinrich A, et al. 2020. Cell Rep. 31:107513. PubMed
  81. Udumula MP, et al. 2021. Mol Metab. 53:101272. PubMed
  82. Karuppuchamy T, et al. 2020. Inflamm Bowel Dis. 216:26. PubMed
  83. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  84. Huppé CA, et al. 2018. Mucosal Immunol. 0.536111111. PubMed
  85. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  86. Lee YJ, et al. 2018. FASEB J. 32:4658. PubMed
  87. Atif SM, et al. 2019. JCI Insight. 4:e125494. PubMed
  88. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  89. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
  90. Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed
RRID
AB_312708 (BioLegend Cat. No. 100505)
AB_312709 (BioLegend Cat. No. 100506)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 4    Revision Date: 10.02.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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