Purified anti-mouse CD370 (CLEC9A, DNGR1) Antibody

Pricing & Availability
Clone
7H11 (See other available formats)
Other Names
DNGR-1, C-type lectin domain family 9 member A, CD370
Isotype
Rat IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
143502 100 µg 185 CHF
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Description

CD370 (CLEC9A), also known as DNGR1, is a 264 amino acid (aa) type II transmembrane glycoprotein which belongs to the C-type lectin superfamily. It contains a 208 aa extracellular region with a single carbohydrate recognition domain (CRD) and a cytoplasmic segment (35 aa) bearing an immunoreceptor tyrosine-based activation motif (ITAM).  In mice, CLEC9A is strictly expressed on CD8a-positive dendritic cells and plasmacytoid dendritic cells. It recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA, ICC, IP, WB - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA2, immunofluorescence microcopy1, immunoprecipitation1, and Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Sancho D, et al. 2008. J. Clin. Invest. 118:2098. (IF, IP, WB)
  2. Joffre OP, et al. 2010. Eur. J. Immunol. 40:1255. (ELISA)
Product Citations
  1. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
Publication Library
RRID
AB_11125169 (BioLegend Cat. No. 143502)

Antigen Details

Structure
Type II transmembrane glycoprotein, consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD)
Distribution

Strictly expressed on CD8α+ dendritic cells, migratory CD103+ dendritic cells, and plasmacytoid dendritic cells

Function
Cross present antigen to CTL
Interaction
Necrotic cells
Ligand/Receptor
Ubiquitous preformed acid-labile protein associated ligand(s)
Cell Type
Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens
Antigen References

Poulin LF, et al. 2012. Blood 119:6052.

Gene ID
232414 View all products for this Gene ID
UniProt
View information about CD370 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 01.15.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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