Purified anti-mouse CD31 Antibody

Pricing & Availability
Clone
MEC13.3 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
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Product Citations
publications
MEC13dot3_Purified_080607
C57BL/6 mouse splenocytes stained with purified MEC13.3, followed by anti-rat IgG FITC
  • MEC13dot3_Purified_080607
    C57BL/6 mouse splenocytes stained with purified MEC13.3, followed by anti-rat IgG FITC
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102501 50 µg 71 CHF
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102502 500 µg 256 CHF
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Polyoma middle T transformed EC line tEnd.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Block, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530).

Application References

(PubMed link indicates BioLegend citation)
  1. Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
  2. Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
  3. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  4. Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
  5. Baldwin HS, et al. 1994. Development 120:2539. (IHC)
  6. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  7. Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  10. Morita R, et al. 2015. PNAS. 112:160. PubMed
  11. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Betterman KL, et al. 2020. J Clin Invest. 130:3315. PubMed
  2. Brenner JS, et al. 2018. Nat Commun. 9:20222. PubMed
  3. Todd L, et al. 2019. J Neuroinflammation. 16:118. PubMed
  4. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  5. Lei L, et al. 2020. Stem Cell Res Ther. 11:137. PubMed
  6. Schnoor M, et al. 2011. J Exp Med. 208:1721. PubMed
  7. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  8. Reglero-Real N, et al. 2021. Immunity. :. PubMed
  9. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  10. Liu X et al. 2019. Immunity. 50(2):317-333 . PubMed
  11. Challa DK, et al. 2019. MAbs. 11:848. PubMed
  12. Marchant C, et al. 2020. Development. 147:00:00. PubMed
  13. Liu Y, et al. 2019. J Cell Physiol. 234:9525. PubMed
  14. Juan T, et al. 2009. Clin Cancer Res. 3.819444444. PubMed
  15. Gao J, et al. 2017. Oncol Lett. 14:2954. PubMed
  16. Dertschnig S, et al. 2020. J Clin Invest. 130:1896. PubMed
  17. Lobo P, et al. 2012. J Immunol. 188:1675. PubMed
  18. Dooley J, et al. 2020. Cell Rep. 32:107880. PubMed
  19. Yang X, et al. 2020. Cell Biosci. 0.488194444. PubMed
  20. Carr MJ, et al. 2019. Cell Stem Cell. 24:240. PubMed
  21. Song A, et al. 2018. Sci Rep. 8:723. PubMed
  22. Woyciechowski S, Hofmann M, Pircher H 2017. Eur J Immunol. 47:244-250. PubMed
  23. Na YR, et al. 2020. Cell Rep. 107643:31. PubMed
  24. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  25. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  26. Baluk P, et al. 2020. Am J Pathol. 190:2355. PubMed
  27. Opzoomer JW, et al. 2021. Sci Adv. 7:eabg9518. PubMed
  28. Crosse EI, et al. 2020. Cell Stem Cell. 27:822. PubMed
  29. Fu H, et al. 2019. Front Immunol. 10:271. PubMed
  30. Arumugam R, et al. 2020. Immunobiology. 151896:225. PubMed
  31. Shiuan E, et al. 2020. F1000Research. 0.525694444. PubMed
  32. Sun W, et al. 2021. Mol Ther. 29:1312. PubMed
  33. Hansmeier NR, et al. 2022. Int J Mol Sci. 23:. PubMed
  34. Cardoso F, et al. 2021. Nature. 597:410. PubMed
  35. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  36. Takahashi K, et al. 2020. J Biol Chem. 295:12559. PubMed
  37. Kuboi Y, et al. 2019. Int Immunol. 31:287. PubMed
  38. Maul A, et al. 2022. Elife. 11:. PubMed
  39. Poulos MG, et al. 2017. J Clin Invest. 127:4163. PubMed
  40. Ohnuki H, et al. 2012. Blood. 119:2688. PubMed
  41. Andreev D, et al. 2020. J Clin Invest. 130:4811. PubMed
  42. Lee C, et al. 2020. J Shoulder Elbow Surg. 719:29. PubMed
  43. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
  44. Sun JX, et al. 2018. Angiogenesis. 8:2407. PubMed
  45. Matsumoto A, et al. 2021. iScience. 24:102839. PubMed
  46. Koch PS, et al. 2021. Front Physiol. 12:722394. PubMed
  47. Zlotoff D, et al. 2011. Blood. 118:1962. PubMed
  48. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  49. Cheung K, et al. 2015. Proc Natl Acad Sci U S A. 112: E5815 - E5824. PubMed
  50. Genoud V, et al. 2018. Oncoimmunology. 7:e1501137. PubMed
  51. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  52. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  53. Zakharova I, et al. 2022. Front Bioeng Biotechnol. 10:772981. PubMed
  54. Storer MA, et al. 2020. Developmental Cell. 52(4):509-524.e9.. PubMed
  55. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  56. Kolb R, et al. 2016. Nat Commun. 7:13007. PubMed
  57. Fierle JK, et al. 2021. Cell Reports Medicine. 2(8):100362. PubMed
  58. Lee C, et al. 2020. J Orthop Res. 1159:38. PubMed
  59. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  60. Baluk P, et al. 2022. Methods Mol Biol. 2441:115. PubMed
  61. Morita R, et al. 2015. Proc Natl Acad Sci U S A. 112:160. PubMed
  62. Huynh H, et al. 2019. Int J Oncol. 54:1123. PubMed
  63. Kabir AU, et al. 2018. JCI Insight. 3. PubMed
  64. Figueiredo CA, et al. 2022. J Neuroinflammation. 19:17. PubMed
RRID
AB_312908 (BioLegend Cat. No. 102501)
AB_312909 (BioLegend Cat. No. 102502)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07.19.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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