Purified anti-LRRK2 Antibody (Previously Covance catalog# SIG-39840)

Pricing & Availability
Clone
MC.028.83.76.242 (See other available formats)
Other Names
Leucine-rich repeat serine/threonine-protein kinase 2, augmented in rheumatoid arthritis 17, AURA17, DARDARIN, PARK8, RIPK7, ROCO2
Previously
Covance Catalog# SIG-39840
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
A_MC.028.83.76.242_PURE_LRRK2_IHCP_060518
IHC staining of purified anti-LRRK2 antibody (clone MC.028.83.76.242) on the formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 25 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_MC.028.83.76.242_PURE_LRRK2_IHCP_060518
    IHC staining of purified anti-LRRK2 antibody (clone MC.028.83.76.242) on the formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 25 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_MC0288376242_PURE_LRRK2_ELISA_060518
    Direct ELISA of purified ant-LRRK2 antibody (clone MC.028.83.76.242). Wells were coated with 100 ng of plate-immobilized recombinant human LRRK2 peptide or sAPP Beta peptide. The wells were then incubated with the primary antibody at 37°C for 60 minutes, followed by incubation with the horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
Cat # Size Price Quantity Avail. Save
808201 100 µL 97 CHF
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Description

Leucine-rich repeat kinase 2 (LRRK2), is a member of the leucine-rich repeat kinase family. This protein also functions as a GTPase. Proteins with leucine-rich regions play a role in protein-protein interactions and mediate assembly of the cell's structural framework (cytoskeleton). Mutations in LRRK2 genes have been associated with late-onset Parkinson's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide immunogen comprising amino acids 2069-2087 of the LRRK2 protein in mice that did not express endogenous LRRK2 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
Direct ELISA - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 25 µg/mL is suggested. For Direct ELISA, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody was generated with support from The Michael J. Fox Foundation. The Michael J. Fox Foundation is dedicated to finding a cure for Parkinson's disease through an aggressively funded research agenda and to ensuring the development of improved therapies for those living with Parkinson's today. To learn more about MJFF, please visit www.michaeljfox.org.

RRID
AB_2564739 (BioLegend Cat. No. 808201)

Antigen Details

Structure
This gene is a member of the leucine-rich repeat kinase family and is encoded by the PARK8 gene. The protein is present mainly in the cytoplasm but can be associated with the mitochondrial outer membrane.
Distribution

Tissue distribution: Lung, Kidney, parathyroid gland, CNS
Cellular distribution: cytoplasm, mitochondrion, nucleus, endoplasmic reticulum, lysosome, Golgi apparatus, endosome

Function
LRRK2 interacts with the C-terminal R2 RING finger domain of parkin. LRRK2 positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. Mutations in this gene have been associated with Parkinson disease.
Biology Area
Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Antigen References
  1. Paisán-Ruíz C, et al. 2004. Neuron. 44(4):595-600.
  2. Alegre-Abarrategui J, et al. 2009. Hum. Mol. Genet. 18(21):4022-34.
  3. Gómez-Suaga P, et al. 2012. Hum. Mol. Genet. 21(3):511–25.
  4. Orenstein SJ, et al. 2013. Nat. Neurosci. 16(4):394–406.
  5. Cherra SJ, et al. 2013. Am. J. Pathol. 182(2):474–84. 
Gene ID
120892 View all products for this Gene ID
UniProt
View information about LRRK2 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 11.04.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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