Purified anti-IRF6 Antibody

Pricing & Availability
Clone
14B2C16 (See other available formats)
Other Names
Interferon regulatory factor 6, IRF-6
Isotype
Mouse IgG2a, κ
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Product Citations
publications
14B2C16_PURE_IRF6_Antibody_1_WB_071816
Total lysates (15 µg protein) from Jurkat (lane 1), HaCaT (lane 2) and NIH3T3 (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-IRF6 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • 14B2C16_PURE_IRF6_Antibody_1_WB_071816
    Total lysates (15 µg protein) from Jurkat (lane 1), HaCaT (lane 2) and NIH3T3 (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-IRF6 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • 14B2C16_PURE_IRF6_Antibody_2_ICFC_051316
    HaCat cells were treated with Fixation Buffer and permeabilized with True-Phos™ Perm Buffer, then stained with purified IRF6 (clone 14B2C16) (filled histogram) or purified mouse IgG2a, κ (open histogram), followed by anti-mouse IgG PE.
  • 14B2C16_PURE_IRF6_Antibody_3_IF_010318
    HaCat cells were stained with purified anti-IRF6 clone (14B2C16) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG antibody and Alexa Fluor® 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
Cat # Size Price Quantity Avail. Save
674502 100 µg 225 CHF
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Description

IRF6 is a member of the IRF family of transcription factors, which are based on the presence of the conserved IRF tryptophan pentad repeat DNA-binding domain. Unlike other IRF transcription factors, IRF6 is not involved in the expression of interferon genes and doesn’t seem to be involved in innate immunity. Data suggests that IRF6 is necessary for appropriate epidermal differentiation and development. Mutations of IRF6 are associated with human craniofacial developmental defects such as cleft lip, palate, tooth agenesis, Van der Woude syndrome, and popliteal pterygium syndrome. IRF6-deficient mice display severe morphological defects in skin and limbs, and result in perinatal lethality as well.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF6 recombinant protein (92-223 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Degen M, et al. 2018. Front Physiol. 9:1703. PubMed
RRID
AB_2565216 (BioLegend Cat. No. 674502)

Antigen Details

Structure
467 amino acids with a predicted molecular weight of 53 kD and contains an IRF trytophan pentad repeat that is responsible for DNA binding.
Distribution

Nucleus and cytoplasm.

Function
IRF6 is a transcriptional activator that regulates proliferation and differentiation of both epithelial cells and keratinocytes. IRF6 is required for normal epidermal development during embryogenesis.
Interaction
Interacts with SERPINB5.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Melath A, et al. 2013. Asian Pac. J. Cancer Prev. 14:6803.
2. Salahshourifar I, et al. 2012. Eur. J. Med. Genet. 55:389.
3. Blackburn J, et al. 2012. Dev. Biol. 365:61.
4. Botti E, et al. 2011. Proc. Natl. Acad. Sci. USA 108:13710.
5. Restivo G, et al. 2011. EMBO 30:4571.
6. Fleming JA, et al. 2009. Mol. Cell Endocrinol. 299:252.

Gene ID
3664 View all products for this Gene ID
UniProt
View information about IRF6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 11.28.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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