Purified anti-IRF5 Antibody

Pricing & Availability
Clone
11F4A09 (See other available formats)
Other Names
Interferon regulatory factor 5 (IRF-5)
Isotype
Mouse IgG2b, κ
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11F4A09_IRF5_Purified_Antibody_1_WB_071916
Total lysates (15 µg protein) from 293E (lane 1), THP-1 (lane 2) and Raw264.7 (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:2500 diluted (0.2 µg/mL) purified anti-IRF5 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • 11F4A09_IRF5_Purified_Antibody_1_WB_071916
    Total lysates (15 µg protein) from 293E (lane 1), THP-1 (lane 2) and Raw264.7 (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:2500 diluted (0.2 µg/mL) purified anti-IRF5 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • 11F4A09_IRF5_Purified_Antibody_2_IP_051816
    Immunoprecipitation of IRF5 from THP-1 cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-IRF5 antibody (clone 11F4A09, lane 4). Western blot was performed using anti-IRF5 antibody (clone 11F4A09).
Cat # Size Price Quantity Avail. Save
652601 25 µg 125 CHF
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652602 100 µg 275 CHF
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Description

IRF-5 is a transcription factor belonging to the interferon regulatory factor family. It controls induction of type I IFN genes as well as inflammatory cytokines and chemokines through TLRs and the MyD88-TRAF6 pathway. Several genetic variations of IRF-5 have been reported as a risk factor of Systemic lupus erythematosus (SLE), which is caused by excess IFNα induction.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF-5 recombinant protein (176-240 aa).
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2-10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Internal testing suggests this clone is not suitable for intracellular flow cytometry.

RRID
AB_2561469 (BioLegend Cat. No. 652601)
AB_2561470 (BioLegend Cat. No. 652602)

Antigen Details

Structure
498 aa with predicted molecular weight of 56 kD. The protein contains a DNA binding domain (IRF tryptophan pentad repeat) and a poly-Glutamate region.
Distribution

Shuttles between the nucleus and the cytoplasm.

Function
The transcription factor IRF-5 participates in the virus-mediated activation of interferon and regulates gene expression induced by cell growth, differentiation, and apoptosis.
Interaction
IKKα phosphorylates IRF-5 and induces formation of IRF-5 dimers. TRAF6 mediates K63-linked ubiquitination of IRF-5, required for nuclear translocation of IRF-5 and regulation of target genes. IRF-5 assemblies with CBP/p300 to initiate transcription.
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Antigen References

1. Balkhi MY, et al. 2010. Cell. Signal. 1:117.
2. Balkhi MY, et al. 2008. Mol. Cell. Biol. 28:7296.
3. Salloum R and Niewold TB. 2011. Transl. Res. 157:326.
4. Barnes BJ, et al. 2003. J. Biol. Chem. 278:16630.
5. Rullo OJ, et al. 2010. Ann. Rheum. Dis. 69:611.

Gene ID
3663 View all products for this Gene ID
UniProt
View information about IRF5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 11.28.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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