Purified anti-IL-27/IL-35 EBI3 (monomer, dimer, heterodimer) Antibody

Pricing & Availability
Clone
J053B5 (See other available formats)
Regulatory Status
RUO
Other Names
Epstein-Barr Virus Induced 3, EBV-Induced Gene 3 Protein, IL-27 Subunit Beta, IL-27B, IL-35 subunit
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
J053B5_Purified_EBI3_Antibody_WB_102214
Western blot analysis of 50 ng of recombinant human IL-27 (lane 1), 50 ng of recombinant mouse IL-27 (lane 2), and 50 ng of recombinant human IL-35 (lane 3) using anti-EBI3 antibody (clone J053B5).
  • J053B5_Purified_EBI3_Antibody_WB_102214
    Western blot analysis of 50 ng of recombinant human IL-27 (lane 1), 50 ng of recombinant mouse IL-27 (lane 2), and 50 ng of recombinant human IL-35 (lane 3) using anti-EBI3 antibody (clone J053B5).
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662602 100 µg 265 CHF
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Description

EBI3 is an IL-12 p40-related protein that can heterodimerized with IL-12 p35 to form IL-35, or with an IL-12 p35-related protein, p28, to form IL-27. It is produced by antigen-presenting cells such as B cells, monocytes, and dendritic cells (DCs) following stimulation through TLR. It can regulate T cell differentiation and proliferation. Both IL-27 and IL-35 are anti-inflammatory cytokines that are able to inhibit Th17 generation, promote Th1 differentiation, increase cytolytic activity of CD8+ T cells, and impact B cell differentiation and immunoglobulin production. IL-35 is also involved in tumorigenesis and is a promising biomarker for assessing tumor progression and prognosis in clinical settings.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse EBI3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564303 (BioLegend Cat. No. 662602)

Antigen Details

Structure
229 amino acids, predicted molecular weight of approximately 25 kD.
Distribution

B cells, monocytes, and dendritic cells.

Function
Pro- and anti-inflammatory.
Interaction
IL-27 p28 subunit and IL-35 p35 subunit.
Ligand/Receptor
WSX-1(TCCR) and gp130.
Cell Type
B cells, Dendritic cells, Monocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Pflanz S, et al. 2002. Immunity 16:779.
2. Takeda A, et al. 2003. J. Immunol. 170:4886.
3. Pflanz S, et al. 2004. J. Immunol. 172:2225.
4. Li MF, et al. 2013. Dev. Comp. Immunol. 41:514.
5. Zeng JC, et al. 2013. Int. J. Clin. Exp. Pathol. 6:1806.
6. Ouyang H, et al. 2014. Am. J. Med. Sci. 348:156.

Gene ID
10148 View all products for this Gene ID
UniProt
View information about IL-27 IL-35 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.14.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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