Purified anti-human/mouse CD49f Antibody

Pricing & Availability
GoH3 (See other available formats)
Regulatory Status
HCDM listed
Other Names
VLA-6 α chain, α6 integrin, integrin α6, ITGA6
Rat IgG2a, κ
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Product Citations
Human peripheral blood lymphocytes stained with purified GoH3, followed by anti-rat IgGs FITC
  • GoH3_Purified_071307
    Human peripheral blood lymphocytes stained with purified GoH3, followed by anti-rat IgGs FITC
  • zGoH3_IF_073012
    MDA-MB-231 breast cancer cells were stained with anti-CD49f (clone GoH3) followed by DyLight™ 649 Goat anti-rat Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
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313602 100 µg 119 CHF
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CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Mouse, Baboon, Capuchin Monkey, Cat, Cow, Chimpanzee, Cynomolgus, Dog, Horse, Rabbit, Sheep, Pig
Antibody Type
Host Species
Mouse mammary tumor cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
ICC, IHC-F, IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin a61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin a6 function in vivo.

Application References

(PubMed link indicates BioLegend citation)
  1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
  2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
  3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
  4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
  5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
  6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
  7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
  8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
  9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
  10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
  11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
  14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
  15. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  3. García–Mariscal A, et al. 2018. Oncogene. 37:847. PubMed
  4. Lowndes M, et al. 2016. Stem Cell Reports. 7:126-137. PubMed
  5. Leelatian N, et al. 2020. eLife. 9:e56879.. PubMed
  6. Heinrich A, et al. 2021. Cell Rep. 37:109885. PubMed
  7. Larsen SB, et al. 2021. Cell Stem Cell. :. PubMed
  8. van der Heide V, et al. 2022. Cell Rep. :110508. PubMed
  9. Sivasubramaniyan K, et al. 2015. Glycobiology. 25: 902-917. PubMed
  10. Engelbrecht E, et al. 2020. eLife. 9:e52690.. PubMed
  11. Casey AE, et al. 2018. J Cell Biol. 217:2951. PubMed
  12. Liu LP et al. 2019. Cell Rep. 27(2):455-466 . PubMed
  13. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  14. Romagnoli M, et al. 2020. Development. 147:. PubMed
  15. Seino T et al. 2018. Cell stem cell. 22(3):454-467 . PubMed
  16. Xi L, et al. 2020. Elife. 9:00. PubMed
  17. Mok KW et al. 2018. Developmental cell. 48(1):32-48 . PubMed
  18. Nanki K, et al. 2018. Cell. 174:856. PubMed
  19. Ishibashi R, et al. 2022. Sci Rep. 12:17775. PubMed
  20. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  21. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
  22. Kawasaki K, et al. 2020. Cell. 183(5):1420-1435.e21. PubMed
  23. Barbar L, et al. 2020. Neuron. 107(3):436-453.e12. PubMed
  24. Doffe F, et al. 2022. Cancers (Basel). 14:. PubMed
  25. Lai K, et al. 2012. Mol Endocrinol. 26:52:00. PubMed
  26. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
AB_345296 (BioLegend Cat. No. 313602)

Antigen Details

Integrin family, associates with β1 or β4, 120 kD

Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells

Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Monocytes, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.

Gene ID
16403 View all products for this Gene ID 3655 View all products for this Gene ID
View information about CD49f on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 08.25.2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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