Purified anti-human LT-α (TNF-β) Antibody

Pricing & Availability
Clone
359-238-8 (See other available formats)
Other Names
Lymphotoxin-α (LT- α), Tumor necrosis factor-β (TNF-β), Coley's toxin, Hemorrhagic factor, Necrosin, Natural killer cytotoxic factor (NKCF), Differentiation inducing factor (DIF), TNFSF-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
503002 500 µg 200 CHF
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Description

Lymphotoxin-α (LT-α), also known as tumor necrosis factor-beta (TNF-β), is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. LT-α possesses a signal peptide sequence and is a secreted protein; however, LT-α is also present on the surface of activated T, B and LAK cells as a complex with LT-β. Bioactive LT-α exists as a homotrimer.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E.coli expressed, recombinant human LT-α.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1,2: The purified 359-238-8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 359-81-11 antibody (Cat. No. 503104) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1,2: The 359-238-8 antibody can neutralize the bioactivity of human LT-α. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human LT-α bioactivity (Cat. No. 503004).

Application References

(PubMed link indicates BioLegend citation)
  1. Meager A, et al. 1987. J. Immunol. Methods 104:31.
  2. Meager A, et al. 1987. Hybridoma. 6:305.
Product Citations
  1. Ong L, et al. 2016. J Cereb Blood Flow Metab. 10.1177/0271678X16654920. PubMed
Publication Library
RRID
AB_315268 (BioLegend Cat. No. 503002)

Antigen Details

Structure
TNF superfamily; trimer; 25 kD (Mammalian)
Bioactivity
Transformed cell cytotoxicity; mediator of inflammatory and immune functions; fibroblast synthesis of GM-CSF, G-CSF, IL-1, collagenase, prostaglandin E2; monocyte terminal differentiation, synthesis of G-CSF; neutrophil chemoattractant, production of reac
Cell Sources
Activated T and B cells, fibroblasts, astrocytes, myeloma, endothelial cells, epithelial cells
Cell Targets
Monocytes, B cells, fibroblasts, neutrophils, osteoclasts, keratinocytes, endothelial cells
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Aggarwal B, et al. Eds. 1992. Tumor necrosis factors:structure, function, and mechanism of action. Marcel Dekker Inc.
3. Bonavida B, et al. Eds. 1990. Tumor necrosis factor:structure, mechanisms of action, role in disease and therapy. Karger, Basel.
4. Paul N, et al. 1987. Annu. Rev. Immunol. 6:407.

Regulation
Type II integral membrane protein, forms heterotrimer with type II integral membrane protein LT-β either as LTα1β2 or LTα2β1; processed secreted form is trimeric
Gene ID
4049 View all products for this Gene ID
UniProt
View information about LT-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.12.2013

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