Purified anti-human LAP (TGF-β1) Antibody

Pricing & Availability
Clone
TW4-6H10 (See other available formats)
Other Names
Latency Associated Peptide (LAP), Transforming growth factor beta 1 (TGF-b1), TGFB1, DPD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_LAP_Human_Antibody_ELISA_021214
Human peripheral blood mononuclear cells stimulated with CD3 (UCHT1), CD28 (CD28.2), and recombinant human IL-2 for 24-hours; and then surface stained with purified TW4-6H10 conjugated with PE (top) or mouse IgG1, κ PE isotype control (bottom), followed by intracelluar staining with FOXP3 (259D) Alexa Fluor® 647 (gated on CD4+ lymphocytes)
  • 1_LAP_Human_Antibody_ELISA_021214
    Human peripheral blood mononuclear cells stimulated with CD3 (UCHT1), CD28 (CD28.2), and recombinant human IL-2 for 24-hours; and then surface stained with purified TW4-6H10 conjugated with PE (top) or mouse IgG1, κ PE isotype control (bottom), followed by intracelluar staining with FOXP3 (259D) Alexa Fluor® 647 (gated on CD4+ lymphocytes)
  • 2_TW4-6H10_PURE_LAP_Antibody_2_061719
    Human platelets were stained with LAP (TGF-β1) (clone TW4-6H10) Purified (filled histogram) or Mouse IgG1, κ Purified isotype control (open histogram) followed by anti-mouse IgG biotin and Streptavidin-PE.
Cat # Size Price Quantity Avail. Save
349702 100 µg 165 CHF
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Description

TGF-β1 (transforming growth factor β1) is a multifunctional cytokine which regulates cellular proliferation and differentiation. It is ubiquitously expressed by many types of cells. Platelets express high level of TGF-β. TGF-β is synthesized as a large protein precursor and then secreted as a complex of TGF-β and LAP (latency-associated peptide), in which LAP noncovalently associates with the dimeric mature TGF-β to prevent its activity.  TGF-β requires activation before it binds to its receptors and exerts functions. It has been reported that LAP-TGF-β binds to the integrins αvβ1, αvβ6, αvβ8, and α8β1 through RGD domain. TGF-β plays an important role in controlling proliferation and differentiation of epithelial cells, endothelial cells, fibroblasts, neurons, osteoclasts, and osteoblasts. TGF-β is believed to be important in the regulation of the development of Treg, Th17, and Th9 cells. A study has shown that LAP is an activated Treg surface marker.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
LAP (TGF-β)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
FC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay and immunofluorescent staining with flow cytometric analysis. For ELISA, a concentration of 0.5 µg/ml of the capture antibody was utilized to generate the example standard curve. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application Notes

ELISA:
To measure human LAP, biotinylated TW4-6H10 (Cat. No. 349707) can be used as a detection antibody in sandwich ELISA format, and paired with the purified TW4-6H10 (Cat. No. 349702) as the capture antibody. Recombinant Human LAP (TGF-β1) (ELISA Std.) (Cat. No. 593409) can be used as the protein standard.
To measure human latent TGF-β, biotinylated TW4-6H10 (Cat. No. 349707) can be used as a detection antibody in sandwich ELISA format, and paired with the LEAF™ purified TW7-7H4 (Cat. No. 361202) as the capture antibody. Recombinant Human Latent TGF-β (ELISA Std.) (Cat. No. 593509) can be used as the protein standard.

Flow Cytometry: The purified and fluorochrome-labeled TW4-6H10 antibodies are useful for immunofluorescent staining and flow cytometric analysis.

Note: For testing human LAP (TGF-β1) in serum, plasma or supernatant, LEGEND MAX™ Human LAP ELISA Kit (Cat. No. 436807 & 436808) is specially developed and recommended. For testing human latent TGF-β, LEGEND MAX™ Human Latent TGF-β ELISA Kit (Cat. No. 432907 & 432908) is recommended.

RRID
AB_10643419 (BioLegend Cat. No. 349702)

Antigen Details

Structure
About 400 amino acids, N-terminal signal peptide is required for secretion from a cell, a pro-region (LAP) and C-terminal region that becomes the mature TGF-β molecule following its release from the pro-region by proteolytic cleavage.
Distribution

Ubiquitously expressed by many kinds of cells

Function
Multifunctional cytokine, regulates cells proliferation, and differentiation
Ligand/Receptor
TGF-βRI, -RII, -RIII, CD105, LTBP, integrins
Cell Type
B cells, Dendritic cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Yi JJ, et al. 2010. Cell 142:144.
2. Tran DQ, et al. 2009. Blood 113:5125.
3. Lu M, et al. 2002. J. Cell. Sci. 115:4641.
4. Khalil N. 1999. Microbes Infect. 1:1255.

Gene ID
7040 View all products for this Gene ID
UniProt
View information about LAP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02.14.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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