Purified anti-human IL-1α Antibody

Pricing & Availability
Clone
364-3B3-14 (See other available formats)
Other Names
Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
500104 500 µg 230 CHF
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The 364-3B3-14 antibody reacts with human interleukin-1α (IL-1α). The 364-3B3-14 antibody is a non-neutralizing antibody.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity*: Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-1α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 200 to 1.56 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1: The purified 364-3B3-14 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5313 antibody (Cat. No. 531304) as the detecting antibody and recombinant human IL-1α (Cat. No. 570009) as the standard. The LEAF™ purified antibody (Cat. No. 500102) is suggested for ELISPOT capture.
Flow Cytometry4: The fluorochrome-labeled 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1α -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Additional reported applications (for the relevant formats) include: immunohistochemistry3. The 364-3B3-14 is a non-neutralizing antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Thorpe R, et al. 1988. Lymphokine Res. 2:119.
  2. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
  3. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  4. Enriquez, J, et al. 2002. Adv. Perit Dial. 18:177.
  5. Lu Y, et al. 2015. PNAS. 112:607. PubMed
Product Citations
  1. Lu Y, et al. 2015. Proc Natl Acad Sci U S A. 112:607. PubMed
Publication Library
RRID
AB_315083 (BioLegend Cat. No. 500104)

Antigen Details

Structure
Cytokine; 17.5 kD (Mammalian)
Bioactivity
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
Cell Sources
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Cell Targets
B cells, T cells, monocytes, dendritic cells
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bomford, R., et al. Eds. 1989. Interleukin-1, inflammation and disease. Elsevier, New York.
3. Brazel, D., et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello, C. 1996. Blood 87:2095.

Regulation
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID
3552 View all products for this Gene ID
UniProt
View information about IL-1alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 11.13.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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