Loading...

Purified anti-human HLA-DRB1 Antibody

Pricing & Availability
Clone
NFLD.D2 (See other available formats)
Regulatory Status
RUO
Other Names
SS1, DRB1, DRw10, HLA-DRB, HLA-DR1B
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
NFLD_Purified_HLA-DRB1_Antibody_FC_1_061314
Human peripheral blood lymphoyctes were stained with CD20 APC and purified anti-human HLA-DRB1 (clone NFLD.D2, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.
  • NFLD_Purified_HLA-DRB1_Antibody_FC_1_061314
    Human peripheral blood lymphoyctes were stained with CD20 APC and purified anti-human HLA-DRB1 (clone NFLD.D2, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.
  • NFLD_Purified_HLA-DRB1_Antibody_FC_2_061314
Cat # Size Price Quantity Check Availability Save
362301 100 µg 323 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

HLA-DRB1, a HLA class II beta chain paralog, is a heterodimer membrane anchored α (DRA) and β chain (DRB). It plays an imporant role in extracellular protein-derived peptide presentation. It is expressed by antigen presenting cells (APC) such as B cells, dendritic cells, and macrophages. The β chain (composed of β1 and β2) is 26-28 kD and contains polymorphisms allowing for variability in peptide binding specificities. DRB1 is expressed by all individuals and its expression is five fold higher than DRB chains 3, 4, or 5. The peptide binding groove can accommodate peptides 10-30 residues long. Thirty-three different sub-types of HLA-DRB1*04 have been described. Of these, HLA-DRB1*04 is associated with an increased incidence of rheumatoid arthritis and is present on a subset of DRB1 molecules.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
L-cell transfectants HLA-DRB1*0401(11th IHW 8115), CFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone NFLD.D2 recognizes a shared epitope (DRB1*70-74:QKRAA and QRRAA) of HLA_DRB1*04.

Application References

(PubMed link indicates BioLegend citation)
  1. Wicks I, et al. 1997. Ann. Rheum Dis. 56:135 (FC)
  2. Patil NS, et al. 2001. J. Immunol. 12:7157 (FC)
  3. Drover S, et al. 1998. Hum. Immunol. 59:77 (FA)
RRID
AB_2563457 (BioLegend Cat. No. 362301)

Antigen Details

Structure
MHC class II cell surface receptor, 30 kD
Distribution
APCs
Function
Presentation of the peptide to T cells leads to activation or suppression of the immune system, which eventually leads to the immune response.
Interaction
CD4+ T cells
Cell Type
Antigen-presenting cells, B cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Scally SW, et al. 2013. J. Exp. Med. 210:2569.
2. Balnyte R, et al. 2013. BMC Neurol. 13:77.
3. Ranasinghe S, et al. 2013. Nat. Med. 19:930.
4. Foo JN, et al. 2013. Am. J. Hum. Genet. 93:167.
5. Balandraud N, et al. 2013. PLoS One. 8:e64108.
6. Sutton VR, et al. 1988. Hum. Immunol. 22:123.
7. Stunz LL, et al. 1989. J. Immunol. 143:3081.

Gene ID
3123 View all products for this Gene ID
UniProt
View information about HLA-DRB1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.16.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account