Purified anti-human CD62E Antibody

Pricing & Availability
Clone
HCD62E (See other available formats)
Other Names
E-selectin, ELAM-1, LECAM-1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HCD62E_Pure_030308
TNF-α-stimulated (6 hours) HUVEC cells stained with purified HCD62E, followed by anti-mouse IgG FITC
  • HCD62E_Pure_030308
    TNF-α-stimulated (6 hours) HUVEC cells stained with purified HCD62E, followed by anti-mouse IgG FITC
Cat # Size Price Quantity Avail. Save
322602 100 µg 100 CHF
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Description

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_536004 (BioLegend Cat. No. 322602)

Antigen Details

Structure
Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution

Highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression); can also be expressed on endothelial cells in the skin, bone marrow and placenta.

Function
Involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand/Receptor
Siayl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported in interact with CD62E
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Collins T, et al. 1991. J. Biol. Chem. 266:2466.
2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238.
3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
4. Lawrence MB, et al. 1993. J. Immunol. 151:6338.
5. Walz G, et al. 1990. Science 250:1132.

Gene ID
6401 View all products for this Gene ID
UniProt
View information about CD62E on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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