Purified anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
A19063A (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1, NCAM-1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_A19063A_PURE_CD56_Antibody_052322
Human paraffin-embedded cerebellum tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD56 (NCAM) (clone A19063A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) (clone Poly4053) (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
  • 1_A19063A_PURE_CD56_Antibody_052322
    Human paraffin-embedded cerebellum tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD56 (NCAM) (clone A19063A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) (clone Poly4053) (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
  • 2_A19063A_PURE_CD56_Antibody_2_052322
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD56 (NCAM) (clone A19063A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) (clone Poly4053) (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
  • 3_A19063A_PURE_CD56_Antibody_WB_052322
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-human CD56 (NCAM) (clone A19063A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • 4_A19063A_PURE_CD56_Antibody_ICC_052322
    ICC staining of purified anti-human CD56 (NCAM) (clone A19063A) on HeLa cells. The cells were fixed and permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 1.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the image was captured with a 40X objective.
  • 5_A19063A_PURE_CD56_Antibody_ICC_2_052322
    ICC staining of mouse IgG2b, κ isotype control (Cat. No. 400301) on HeLa cells. The cells were fixed and permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 1.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the image was captured with a 40X objective.
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380702 100 µg 127 CHF
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.1 - 10.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922611 (BioLegend Cat. No. 380702)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparan sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  2. Pachnio A, et al. 2016. PLoS Pathog. 12:e1005832. PubMed
  3. Wouters K, et al. 2017. Sci Rep. 7:42665. PubMed

 

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.23.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
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  • Purified anti-human CD56 (NCAM)

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