Purified anti-human CD49e Antibody

Pricing & Availability
NKI-SAM-1 (See other available formats)
Regulatory Status
Other Names
α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
Mouse IgG2b, κ
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Product Citations
Human peripheral blood lymphocytes stained with purified NKI-SAM1, followed by anti-mouse IgG FITC
  • NKI-SAM1_Pure_122007
    Human peripheral blood lymphocytes stained with purified NKI-SAM1, followed by anti-mouse IgG FITC
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328002 100 µg 118 CHF
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CD49e is a type I integral membrane glycoprotein, known as α5 integrin, VLA-5 α chain. It forms noncovalent heterodimer with integrin β1 (CD29). CD49e contains two disulfide-linked subunits of 135 kD and 24 kD, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Host Species
U937 cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IP, ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and inhibition of a5 integrin mediated cell adhesion2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 328004).

Application References

(PubMed link indicates BioLegend citation)
  1. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  2. Orecchia A, et al. 2003. J. Cell Sci. 116:3479. (Block)
Product Citations
  1. Park S, et al. 2019. Sci Rep. 9:13286. PubMed
  2. Sivasubramaniyan K, et al. 2015. Glycobiology. 25: 902-917. PubMed
  3. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  4. Burckhardt CJ, et al. 2021. Developmental Cell. 56(8):1164-1181.e12. PubMed
AB_893363 (BioLegend Cat. No. 328002)

Antigen Details

Integrin family, associates with integrin ß1 (CD29), 135 kD and 25 kD in reduced condition, 155 kD (unreduced)

Thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, some myeloid leukemia cells

Adhesion, regulation of cell survival and apoptosis
Fibronectin, neural adhesion molecule L1
Cell Type
Endothelial cells, Leukemia, Lymphocytes, Mesenchymal Stem Cells, Osteoclasts, Thymocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1.Tran Van Nhieu GT and Isberg RR. 1991. J. Biol. Chem. 266:24367.
2. Isaji T, et al. 2006.J. Biol. Chem. 281:33258.

Gene ID
3678 View all products for this Gene ID
View information about CD49e on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD49e Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD49e NKI-SAM-1 FC, IP, ELISA
FITC anti-human CD49e NKI-SAM-1 FC
PE anti-human CD49e NKI-SAM-1 FC
APC anti-human CD49e NKI-SAM-1 FC
Ultra-LEAF™ Purified anti-human CD49e NKI-SAM-1 FC, IP, ELISA, Block
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD49e

  • FITC anti-human CD49e

  • PE anti-human CD49e

  • APC anti-human CD49e

  • Ultra-LEAF™ Purified anti-human CD49e


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