Purified anti-human CD25 Antibody

Pricing & Availability
Clone
M-A251 (See other available formats)
Regulatory Status
RUO
Workshop
IV A053
Other Names
IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M-A251_Purified_CD25_Antibody_FC_010813
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with purified CD25 (clone M-A251) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • M-A251_Purified_CD25_Antibody_FC_010813
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with purified CD25 (clone M-A251) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
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356101 25 µg 50,00 CHF
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356102 100 µg 110,00 CHF
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PHA-induced lymphocyte cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections.1

The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.

Application References

(PubMed link indicates BioLegend citation)
  1. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
Product Citations
  1. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  2. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  3. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  4. Joas S, et al. 2020. Cell Rep. 30:2261. PubMed
  5. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  6. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  7. Neidleman J, et al. 2020. Elife. 9:00. PubMed
  8. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  9. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
  10. Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
  11. Kasatskaya SA, et al. 2020. Elife. :9. PubMed
  12. Neidleman J, et al. 2021. Cell Rep. 36:109414. PubMed
RRID
AB_2561751 (BioLegend Cat. No. 356101)
AB_2561752 (BioLegend Cat. No. 356102)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
Distribution

Activated T and B cells, monocytes/macrophages, Tregs

Interaction
Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01.22.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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