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Purified anti-human CD220 Antibody

Pricing & Availability
Clone
B6.220 (See other available formats)
Regulatory Status
RUO
Other Names
Insulin Receptor
Isotype
Mouse IgG2b, κ
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Product Citations
publications
B6.220_Pure_082911
Human peripheral blood monocytes were stained with purified CD220 (clone B6.220) (filled histogram) or mouse IgG2b, κ isotype control, followed by biotinylated anti-mouse IgG and Sav-PE (open histogram).
  • B6.220_Pure_082911
    Human peripheral blood monocytes were stained with purified CD220 (clone B6.220) (filled histogram) or mouse IgG2b, κ isotype control, followed by biotinylated anti-mouse IgG and Sav-PE (open histogram).
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352602 100 µg 136 CHF
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Description

CD220, also known as insulin receptor, is a type I transmembrane receptor tyrosine kinase composed of two extracellular α-subunits and two transmembrane β-subunits. Binding insulin, the insulin receptor forms a heterotetramer of two units to induce autophosphorylation and activation of the tyrosine kinase activity of the receptor.  Activation of insulin receptor leads to subsequent downstream signaling in metabolic regulation, inducing glucose uptake, cell growth, differentiation, and apoptosis. Gene mutation in the insulin receptor or decreased insulin receptor signaling leads to insulin-resistant diabetes mellitus and noninsulin-dependent diabetes mellitus (diabetes mellitus type 2). Most normal cells constitutively express insulin receptors. In hematopoietic cells, insulin receptor is constitutively expressed on monocytes and selectively expressed on activated lymphocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human insulin receptor/freund's adjuvant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  2. Müller M, et al. 2021. Nat Commun. 12:7036. PubMed
RRID
AB_10896052 (BioLegend Cat. No. 352602)

Antigen Details

Structure
Consist of two alpha subunits and two beta subunits; the beta subunit passes through the cellular membrane and is linked by disulfide bonds.
Distribution

Hematopoietic and non hematopoietic cells.

Function
Regulates cell metabolism and growth.
Interaction
Ectonucleotide pyrophosphatase/phosphodiesterase, PTPN11, GRB10, GRB7, PRKCD, IRS1, SH2B1 and Mothers against decapentaplegic homolog 2.
Ligand/Receptor
Insulin
Biology Area
Cell Biology, Immunology, Protein Synthesis, Signal Transduction
Molecular Family
CD Molecules, Protein Kinases/Phosphatase
Antigen References

1. Viardot A, et al. 2006. Endocrinology 148:346.
2. Ward CW, et al. 2009. Bioessays 31:422.
3. Brindle NP, et al. 1990. Biochem. J. 268:615.

Gene ID
3643 View all products for this Gene ID
UniProt
View information about CD220 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08.17.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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