Purified anti-human CD210 (IL-10 R) Antibody

Pricing & Availability
Clone
3F9 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70502
Other Names
IL-10 Receptor, IL-10R
Isotype
Rat IgG2a, κ
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Product Citations
publications
3F9
Whole blood lymphocytes stained with purified 3F9, then detected with biotinylated anti-rat IgG followed by Sav-PE
  • 3F9
    Whole blood lymphocytes stained with purified 3F9, then detected with biotinylated anti-rat IgG followed by Sav-PE
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308802 100 µg 95,00 CHF
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Description

CD210, also known as the IL-10 receptor, is a 90-110 kD protein expressed on T cells, B cells, NK cells, monocytes and macrophages. CD210 belongs to the class II cytokine receptor family which includes the IFN-γ receptor (CDw119), the IFN-α/β receptor (CD118) and tissue factor (CD142). The IL-10 receptor is involved in signal transduction by inducing phosphorylation of STAT1a and STAT3 and by inducing activation of Jak1 and Tyk.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
shIL-10R
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro blocking1-3 of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. No. 308817 and 308818).

Application References

(PubMed link indicates BioLegend citation)
  1. Liu Y, et al. 1997. J. Immunol. 158:604. (Immunogen, IP, Block)
  2. Levings MK, et al. 2005. Blood 105:1162. (Block)
  3. Goodier MR, et al. 2000. J. Immunol. 165:139. (Block)
  4. Huang YH, et al. 2009. J. Leukoc. Biol. 86:273. PubMed
  5. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  6. Liu BS,et al. 2011. J Leukoc Biol. 89:981. PubMed
  7. Joffe M, et al. 2012. Int Immunol. 24:447. PubMed
  8. MacDonald KP, et al. 1999. J. Immunol. 163:5599. (epitope)
Product Citations
  1. Ni G, et al. 2019. PLoS One. 14:e0213813. PubMed
  2. Svajger U, et al. 2019. Front Immunol. 2.240972222. PubMed
  3. Huang Y, et al. 2009. J Leukoc Biol. 86:273. PubMed
  4. Liu B, et al. 2011. J Leukoc Biol. 89:981. PubMed
  5. Joffe M, et al. 2012. Int Immunol. 24:447. PubMed
  6. Claassen M, et al. 2012. PLoS One. 7:e49389. PubMed
  7. Tang X, et al. 2020. Sci Adv. 6:eaaz0374. PubMed
  8. Jenkins BR, et al. 2021. The FASEB Journal. 35(6):e21551. PubMed
RRID
AB_314734 (BioLegend Cat. No. 308802)

Antigen Details

Structure
Ig superfamily, class II cytokine receptor family, transmembrane glycoprotein, 90-110 kD
Distribution

T cells and B cells, NK cells, monocytes, macrophages

Function
Activates STAT1, STAT3, Jak1, Tyk
Ligand/Receptor
IL-10
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Kotenko S. 2002. Cytokine Growth Factor Rev. 13:223.
2. Trinchieri G. 2003. Nat. Rev. Immunol. 3:133.

Gene ID
3587 View all products for this Gene ID
UniProt
View information about CD210 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12.17.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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