Purified anti-human CD199 (CCR9) Antibody

Pricing & Availability
Clone
L053E8 (See other available formats)
Other Names
GPR 9-6, GPR28, CDw199, Chemokine Receptor 9
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
L0538_Purified_CCR9_Antibody_FC_070313
Human acute lymphoblastic leukemia cell line MOLT-4 was stained with purified CCR9 (clone L053E8, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • L0538_Purified_CCR9_Antibody_FC_070313
    Human acute lymphoblastic leukemia cell line MOLT-4 was stained with purified CCR9 (clone L053E8, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
Cat # Size Price Quantity Avail. Save
358902 100 µg 285 CHF
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Description

Human CD199, also known as CCR9, is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in the gut-associated immune response. It is highly expressed on different stages of thymocytes and upregulated on CD4+ CD8+ cells. Expression of CCR9 on γ/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and the generation of gut-specific immunological memory.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cells transfected with human CCR9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2562298 (BioLegend Cat. No. 358902)

Antigen Details

Structure
A member of G-protein coupled receptors which contains seven transmembrane domains. Human CCR9 has above 80% amino acid sequence homology with its mouse counterpart.
Distribution

CD4+CD8+ thymocytes, γ/δ T cells, subset of memory T cells

Function
May direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory; co-receptor for HIV-1
Ligand/Receptor
CCL25
Cell Type
T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Zaballos A, et al. 1999. J. Immunol. 162:5671.
2. Wurbel MA, et al. 2007. J. Immunol. 178:7598.
3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73.

Gene ID
10803 View all products for this Gene ID
UniProt
View information about CD199 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 07.16.2013

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