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Purified anti-human CD171 (L1CAM) Antibody

Pricing & Availability
Clone
L1-OV198.5 (See other available formats)
Regulatory Status
RUO
Other Names
CAML1, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, S10, SPG1, L1CAM, Neural cell adhesion molecule L1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_L1dashOV198point5_PURE_CD171_Antibody_1_FC_041216
M21 cells were stained with purified CD171 (clone L1-OV198.5, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 1_L1dashOV198point5_PURE_CD171_Antibody_1_FC_041216
    M21 cells were stained with purified CD171 (clone L1-OV198.5, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 2_L1-OV198dot5_PURE_CD171_Antibody_ICC_032218
    HeLa cells were fixed with 2% PFA for 10 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were stained with 2 µg/mL anti-CD171 Antibody (clone L1-OV198.5) followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG in blocking buffer for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 3_L1dashOV198point5_PURE_CD171_Antibody_2_WB_092116
    Total cell lysates (15 µg protein) from HeLa, HepG2 and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-CD171 Antibody (clone L1-OV198.5). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. HDAC1 Antibody was used as loading control.
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371602 100 µg 276 CHF
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Description

CD171, also known as L1CAM or L1, is a transmembrane glycoprotein. It is a 200-220 kD neuronal cell adhesion molecule and a member of the L1 protein family belonging to the immunoglobulin superfamily.

This cell adhesion molecule plays an important role in nervous system development, including neuron-neuron adhesion, signal transduction, axon guidance, cell migration and differentiation. CD171 also has a strong implication in treatment-resistanct cancers. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SKOV-3 ovarian carcinoma cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, WB - Verified
IHC-F, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  2. Kondo N, et al. 2020. Cancers (Basel). 12:00. PubMed
RRID
AB_2616786 (BioLegend Cat. No. 371602)

Antigen Details

Structure
200-220 kD. Several immunoglobulin-like domains and fibronectin-like repeats (type III), linked via a single transmembrane sequence to a conserved cytoplasmic domain.
Distribution

Tetanus-toxin positive neurons, endothelial cells, certain epithelial cells, reticular fibroblasts, colon and breast carcinomas, colon melanoma, tumor cells of neuronal and mesothelial origin.

Function
Adhesion molecule. Homotypic and heterotypic cell-cell interaction in neuronal myelination, neurite outgrowth and regeneration. Axonal guidance, neuronal migration.
Ligand/Receptor
Homophilic interaction with itself and heterophilic binding to integrins, other cell adhesion molecules, extracellular matrix molecules.
Cell Type
Endothelial cells, Epithelial cells, Fibroblasts, Neurons
Biology Area
Cancer Biomarkers, Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Wolterink S, et al. 2010. Cancer Res. 70(6):2504.
2. Huszar M, et al. 2006. Human Pathology 37(8):1000.
3. Kaifi JT, et al. 2006. Anticancer Res. 26(2A):1167.
4. Kaifi JT, et al. 2006. Mod. Pathol. 3:399.
5. Meier F, et al. 2006. Int. J. Cancer 119(3)549.
6. Stoeck A, et al. 2006. Biochem. J. 393:609.
7. Gutwein P, et al. 2005. Clin. Cancer Res. 11(7)2492.

Gene ID
3897 View all products for this Gene ID
UniProt
View information about CD171 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09.21.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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