Purified anti-human CD116 Antibody

Pricing & Availability
4H1 (See other available formats)
Regulatory Status
Other Names
GM-CSFRα chain
Mouse IgG1, κ
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Product Citations
Human peripheral blood monocytes stained with 4H1 FITC
  • 4H1
    Human peripheral blood monocytes stained with 4H1 FITC
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305902 100 µg 85,00 CHF
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CD116 is a 70-85 kD α chain of the GM-CSF receptor. It combines with CDw131 β chain to form the high affinity GM-CSF receptor. A soluble form of CD116, which binds GM-CSF with a relatively low affinity, has been identified. In addition, an alternatively spliced form of CD116 with an altered cytoplasmic tail has been described. CD116 is expressed on various myeloid cells including monocytes, macrophages, neutrophils, eosinophils, dendritic cells and their precursors, fibroblasts, and endothelial cells. CD116 is expressed on myeloid leukemias, osteogenic sarcoma cell lines, osteoblast-like cells and breast and lung carcinoma cell lines.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, African Green
Antibody Type
Host Species
Human GM-CSFRα transfected COS cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IP, WB, IHC-P, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting1, and immunohistochemical staining of acetone-fixed frozen tissue sections4 and paraffin-embedded tissue sections5.

Application References

(PubMed link indicates BioLegend citation)
  1. Stomski FC, et al. 1998. J. Biol. Chem. 273:1192. (WB)
  2. McClure B, et al. 2001. Blood 98:3165. (IP)
  3. Guthridge MA, et al. 2004. Blood 103:820.
  4. Xiong S, et al. 2013. J. Clin. Invest. 123:4264. (IHC)
  5. Sawada H, et al. 2014. J. Exp. Med. 211:263. (IHC).
Product Citations
  1. Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
  2. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  3. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  4. Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed
  5. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  6. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  7. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  8. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
AB_314568 (BioLegend Cat. No. 305902)

Antigen Details

Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70-85 kD

Monocytes, granulocytes, dendritic cells, endothelial precursors

Myeloid hematopoietic cell proliferation, differentiation
Cell Type
Dendritic cells, Endothelial cells, Granulocytes, Monocytes
Biology Area
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Miyajima A, et al. 1993. Blood 82:1960.

Gene ID
1438 View all products for this Gene ID
View information about CD116 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04.24.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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