Purified anti-hnRNPK Antibody

Pricing & Availability
Clone
F45P9C7 (See other available formats)
Other Names
Heterogeneous nuclear ribonucleoprotein K, DC-stretch binding protein, Transformation upregulated protein (TUNP), hnRNP K, hnRNP-K
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
A_F45P9C7_PURE_hnRNPK_WB_050118
Total lysates (15 µg protein) from HepG2, Daudi and UMR106 (rat) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-hnRNPK antibody (clone F45P9C7). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse IgG secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • A_F45P9C7_PURE_hnRNPK_WB_050118
    Total lysates (15 µg protein) from HepG2, Daudi and UMR106 (rat) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-hnRNPK antibody (clone F45P9C7). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse IgG secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • B_F45P9C7_PURE_hnRNPK_Antibody_WB_081616
    Total cell lysate from HeLa cells (lane 1, 15 µg), 293E cells (lane 2, 15 µg), U87MG (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-hnRNPK antibody (clone F45P9C7). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
Cat # Size Price Quantity Avail. Save
634601 25 µg 95 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
634602 100 µg 195 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

hnRNP K (Heterogeneous nuclear ribonucleoprotein K) is a pre-mRNA binding protein and a component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. This protein containing KH domains and is ubiquitously expressed in the cytoplasm and the nucleus with a predicted molecular weight approximately 51 kD. hnRNP K expression and function are increased by epidermal growth factor (EGF). hnRNP K acts as transcriptional regulator and functions in nucleocytoplasmic shuttling, and is modified by phosphorylation (PKC δ, c-Src) and acetylation. Clone F45P9C7 has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat, rabbit and chicken hnRNP K.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ovalbumin-conjugated synthetic Peptide SVKQYSGKFF
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Carpenter B, et al. 2006. Br J Cancer. 95:921. PubMed
  2. Carpenter B, et al. 2007. Br J Cancer. 97:574. PubMed
  3. Alnabulsi A, et al. 2009. Methods of cancer diagnosis, therapy and diagnosis. 4:25.
Product Citations
  1. Carpenter B, et al. 2006. Br J Cancer. 95:921. PubMed
  2. Roychoudhury P, Chaudhuri K 2007. Br J Cancer. 97:574. PubMed
RRID
AB_2120365 (BioLegend Cat. No. 634601)
AB_2120361 (BioLegend Cat. No. 634602)

Antigen Details

Structure
Heterogenous nuclear ribonucleoprotein family, KH domains; 51 kD
Distribution

Ubiquitously expressed cytoplasmic and nuclear protein

Function
Pre-mRNA binding protein, component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. Acts as transcriptional regulator and functions in nucleocytoplasmic shuttling
Interaction
Interacts with Csk, NCK2, Vav1, ITK, CEBP β, c-Src, Fyn, Lyn, Hepatitis virus core protein
Modification
Phosphorylation (PKC δ, c-Src), Acetylation, Methylation
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Matunis MJ, et al. 1992. Mol. Cell. Biol. 12:164.
2. Dejgaard K, et al. 1994. J. Mol. Biol. 236:33.
3. Mandal M, et al. 2001. J. Biol. Chem. 276:9699

Regulation
Epidermal growth factor (EGF) can increase expression and function
Gene ID
3190 View all products for this Gene ID
UniProt
View information about hnRNPK on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 05.01.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Forgot your password? Reset password?
Create an Account