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Purified anti-FOXO3 Antibody

Pricing & Availability
Clone
W15111A (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box O3, FKHRL1, FOXO3A
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
W15111A_PURE_FOXO3_Antibody_WB_111617
Total lysates (15 µg protein) from HEK293T and HEK293T/FOXO3 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1.0 µg/mL) purified anti-FOXO3 antibody, clone W15111A. Proteins were visualized by chemiluminescence detection using HRP-conjugated goat anti-mouse-IgG secondary antibody (Cat. No. 405307) at a 1:3000 dilution. Equal protein loading was confirmed using Direct Blot™ HRP β-Actin (Cat. No. 648807) at a 1:5000 dilution. Lane M: Molecular weight ladder.
  • W15111A_PURE_FOXO3_Antibody_WB_111617
    Total lysates (15 µg protein) from HEK293T and HEK293T/FOXO3 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1.0 µg/mL) purified anti-FOXO3 antibody, clone W15111A. Proteins were visualized by chemiluminescence detection using HRP-conjugated goat anti-mouse-IgG secondary antibody (Cat. No. 405307) at a 1:3000 dilution. Equal protein loading was confirmed using Direct Blot™ HRP β-Actin (Cat. No. 648807) at a 1:5000 dilution. Lane M: Molecular weight ladder.
  • W15111A_PURE_FOXO3_Antibody_WB_2_111617
    Total lysates (15 µg protein) from HEL (human), THP-1 (human, negative control) and NIH3T3 (mouse) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg/ml) purified anti-FOXO3 antibody, clone W15111A. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • W15111A_PURE_FOXO3_Antibody_WB_3_111617
    Total lysates (15, 7, 3 µg protein) from HEL cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-FOXO3 antibody, clone W15111A. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
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686302 100 µg 288 CHF
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Description

The Fork-head box O (FOXO) family of transcription factors is comprised of four family members; FOXO1, FOXO3, FOXO4, and FOXO6. FOXO3 was originally identified as a tumor suppressor and has been shown to regulate several cellular processes, such as cell cycle arrest, apoptosis, autophagy, longevity, and stress response. FOXO3 activity is determined by its post-translational modifications and subcellular localization. FOXO3 is inactivated by cytoplasmic retension through AKT/PI3K signaling pathways. FOXO3 inactivation has been associated with cell proliferation and tumorigenesis. In contrast, over-expression of FOXO3 leads to inhibition of tumor growth. FOXO3 also plays an important role in the development of regulatory T cells. FOXO3-deficient mice showed organ inflammation phenotype and increased production of proinflammatory cytokines during viral infection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human FOXO3 recombinant protein (424-673 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2616882 (BioLegend Cat. No. 686302)

Antigen Details

Structure
673 amino acids with a predicted molecular weight of 71.3 kD. Contains a fork-head domain responsible for DNA binding.
Distribution

Cytoplasm and nucleus.

Function
FOXO3 is a transcription factor, participating in the regulation of cell cycle arrest, apoptosis, autophagy, longevity, and stress response.
Interaction
Interacts with SIRT2, 14-3-3 beta, 14-3-3 zeta, STK4, PIM1, and DDIT3.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Morris BJ, et al. 2015. Gerontology 61:515.
2. Larsen SK, et al. 2014. Oncoimmunology 3:e953411.
3. Eijkelenboom A, et al. 2013. Cell Rep. 5:1664.
4. Yeo H, et al. 2013. EMBO J. 32:2589.
5. Pelosi E, et al. 2013. Nat. Commun. 4:1843.
6. Litvak V, et al. 2012. Nature 490:421.

Gene ID
2309 View all products for this Gene ID
UniProt
View information about FOXO3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 11.30.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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