Purified anti-Filaggrin Antibody

Pricing & Availability
Clone
Poly19058 (See other available formats)
Other Names
Epidermal filaggrin
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly19058_PURE_Filaggrin_Antibody_IHCF_062216
IHC staining of anti-Filaggrin antibody (Poly19058) on frozen human skin tissue. The tissue was incubated with the primary antibody at 2 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
  • Poly19058_PURE_Filaggrin_Antibody_IHCF_062216
    IHC staining of anti-Filaggrin antibody (Poly19058) on frozen human skin tissue. The tissue was incubated with the primary antibody at 2 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
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Cat # Size Price Quantity Avail. Save
905804 100 µg 324 CHF
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Description

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Cross Reactivity: Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This monospecific polyclonal antibody was raised against a peptide corresponding to residues 24-39 in the partial sequence of filaggrin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Wallace L, et al. 2012. J. Cell Sci. 125:1750.
  2. Bayo P, et al. 2008. Endocrinology 149:1377.
  3. Hu Y, et al. 2001. Nature 410:710.
  4. Yuspa SH, et al. 1989. J. Cell Biol. 109:1207.
  5. Liang Y. 2011. Patholog. Res. Int. 2011:936794. (IHC) PubMed
  6. Roop DR, et al. 1984. J. Biol. Chem. 259:8037.
Product Citations
  1. Noguchi S, et al. 2019. Commun Biol. 2:37. PubMed
  2. Lee W, et al. 2019. Nutrients. 11. PubMed
RRID
AB_2616957 (BioLegend Cat. No. 905804)

Antigen Details

Structure
Filaggrin is a 44 kD protein.
Distribution
Cytoskeleton and nucleus.
Function
Mutations in this gene are associated with ichthyosis vulgaris.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure
Gene ID
2312 View all products for this Gene ID
UniProt
View information about Filaggrin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06.22.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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