Purified anti-FGF-basic Antibody

Pricing & Availability
A15021A (See other available formats)
Regulatory Status
Other Names
Fibroblast growth factor 2 (Fgf-2), Heparin-Binding Growth Factor 2, Basic Fibroblast Growth Factor (BFGF), Fgfb, bFGF
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
  • 1_A15021A_PURE_FGFbasic_Antibody_1_WB_070716
  • 2_A15021A_PURE_FGFbasic_Antibody_2_WB_070716
    Western blot analysis of 50ng recombinant human FGF-basic (lane 1, Ala144-Ser288), 50ng human FGF-basic (lane 2, Ala135-Ser288), 50ng recombinant mouse FGF-basic (lane 3), 15µg U87-MG untreated (lane 4) and 15µg U87-MG treated with Brefeldin A for six hours (lane 5). Purified anti-FGF-basic (clone A15021A) antibody was used, followed by HRP goat anti-mouse IgG secondary antibody. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
  • 3_A15021A_PURE_FGF-basic_Antibody_IF_010818
    U87-MG cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL purified anti-FGF-basic (clone A15021A) antibody for two hours at room temperature followed by Alexa Fluor® 594 conjugated goat anti-mouse IgG (red) for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
688502 100 µg 305 CHF
Check Availability

Need larger quantities of this item?
Request Bulk Quote

FGF-basic, also known as FGFb and FGF-2, is a member of the fibroblast growth factor (FGF) family which includes 23 members. FGFb is expressed in almost all tissues and plays an important role in a variety of normal and pathological processes, including development, wound healing, and neoplastic transformation. FGFb is mitogenic for many cell types, both epithelial and mesenchymal. FGFb shows potent angiogenic activity and has been implicated in tumor angiogenesis. In prostate, bladder, and renal cancers, FGFb regulates the induction of metalloproteinases (MMP) that degrade extracellular matrix proteins, thus facilitating tumor metastasis. FGFb binds to a family of four distinct, high affinity tyrosine kinase receptors, designated FGFR-1 to -4. In addition, FGFb binds to the ECM, and heparan sulfate (HS) is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to explain, at the molecular level, how HS/heparin enables FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface, although there is controversy regarding the exact manner by which this occurs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Host Species
Human FGF-basic, amino acids Ala144-Ser288 (Accession# NM_002006) was expressed in E. coli.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

WB - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

AB_2629595 (BioLegend Cat. No. 688502)

Antigen Details

Different isoforms with the predicted molecular weight of approximately 18 - 34kD.

Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, and epithelial cells.

FGF-basic is a potent angiogenic factor, and plays a key role in various physiological and pathological conditions, including embryonic development, wound repair, inflammation, and tumor growth.
Fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, astrocytes, oligodendrocytes, and smooth muscle cells.
FGFR-1 (flg), FGFR-2 (bek, K-sam), FGFR3, and FGFR-4 (flg-2); low affinity coreceptor heparin sulfate and heparin sulfate proteoglycans required for full activity.
Cell Type
Monocytes, Epithelial cells, Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Rusnati M, Presta M. 2007. Curr. Pharm. Des. 13:2025.
2. Chaffer CL, et al. 2007. Differentiation 75:831.
3. Cronauer MV, et al. 2003. Eur. Urol. 43:309.
4. Shimizu A, et al. 2001. J. Biol. Chem. 276:11031.
5. Mohammadi M, et al. 2005. Curr. Opin. Struct. Biol. 15:506.
6. Chiou M, et al. 2006. Longaker MT Biochem. Biophys. Res. Commun. 343:644.

Gene ID
2247 View all products for this Gene ID
View information about FGF-basic on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.07.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-FGF-basic


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account