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Purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3) Antibody (Previously Covance catalog# SIG-38710)

Pricing & Availability
Clone
C219 (See other available formats)
Regulatory Status
RUO
Other Names
Multidrug resistance protein 3, P-glycoprotein 3, multiple drug resistance 3, ATP-binding cassette sub-family B member 4, P glycoprotein 3/multiple drug resistance 3
Previously
Signet Catalog# 8710-01
Covance Catalog# SIG-38710
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a-C219_Purified_C219_Antibody_IHC_032519_updated
IHC staining of purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3antibody (clone C219) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with a 1:20 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • a-C219_Purified_C219_Antibody_IHC_032519_updated
    IHC staining of purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3antibody (clone C219) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with a 1:20 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • b-C219_PURE_CD243-ABCB4_Antibody_2_031820
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1:100 dilution of purified anti-CD243/ABCB4 antibody (clone C219) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • c-C219_PURE_CD243-ABCB4_Antibody_3_031820
    Whole cell extracts (15 µg total protein) from the indicated cell lines and tissue were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1:100 dilution of purified anti-CD243/ABCB4 antibody (clone C219) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
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903701 1 mL 604 CHF
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Description

Clone C219 recognizes an internal, highly conserved amino acid sequence (VQEALD & VQAALD) found in both MDR1 and MDR3 isoforms of P-glycoprotein. The specific binding of the clone C219 was stronger in epitope sequence VQAALD (N-Terminal fragment) than the epitope sequence VQEALD (C-terminal fragmant) (Georges E., et al., 1990). Analysis of p-glycoprotein expression in skeletal and cardiac muscle revealed that C219 cross reacts with a 200kDa protein that migrates in the same position as myosin. C219 has also been reported to detect the 185kDa c-erbB2 protein (Liu, et al., 1997). Care should be taken in the interpretation of true positive P-glycoprotein expression when using myosin-containing tissues or tissues known to have p185 c-erbB2 expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Dog, Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was made against SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
IHC-F, ICC, FC, WB, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:20 - 1:40 is suggested.  For western blotting, the suggested use of this reagent is a 1:100 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hisano Y, et al. 2011. J. Biol. Chem. 286: 1758. (WB)
  2. Beck WT, et al. 1996. Cancer Res. 56:3010. (IHC-P, IHC-F, ICC, FC) PubMed
  3. Evans CH, Baker PD. 1992. Cancer Res. 52: 5893. (WB, FC)
  4. Toth K, et al. 1992. Am J Pathol. 140:1009. (IHC-P, ICC, WB)
  5. Finstad CL, et al. 1991. J Histochem Cytochem. 39:1603. (FC, WB, IHC-P
  6. Georges E, et al. 1990. PNAS. 87:152. (ELISA, IHC-P)
  7. Duan Z, et al. 2014. BMC Cancer. 14:681. (WB)
  8. Inui N, et al. 2012. J Gerontol A Biol Sci Med Sci. 67:1049. (FC, IF)
  9. Erickson MA, et al. 2012. J Neuroinflammation. 9:150. (WB)
  10. Rao VV, et al. 1994. Cancer Res. 54:1536. (WB) PubMed
  11. Lee JJ, et al. 1996. Cancer. 77:1892. (IHC) PubMed
Product Citations
  1. Giddings EL, et al. 2021. Nat Commun. 12:2804. PubMed
  2. Lee J, et al. 1996. Cancer. 77:1892-1898. PubMed
  3. Afrouzian M, et al. 2018. Biochem Pharmacol. 156:467. PubMed
  4. Sophie Kang S, et al. 2016. PLoS One. 11:e0165638. PubMed
  5. Beck W, et al. 1996. Cancer Res. 56:3010-3020. PubMed
  6. Song YK, et al. 2019. J Pharmacol Exp Ther. 368:79. PubMed
RRID
AB_2565033 (BioLegend Cat. No. 903701)

Antigen Details

Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
5244 View all products for this Gene ID
UniProt
View information about CD243/ABCB4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 03.18.2020

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3)
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