Purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3) Antibody (Previously Covance catalog# SIG-38710)

Pricing & Availability
Clone
C219 (See other available formats)
Other Names
Multidrug resistance protein 3, P-glycoprotein 3, multiple drug resistance 3, ATP-binding cassette sub-family B member 4, P glycoprotein 3/multiple drug resistance 3
Previously
Signet Catalog# 8710-01
Covance Catalog# SIG-38710
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
C219_Purified_C219_Antibody_IHC_032519_updated.png
IHC staining of purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3antibody (clone C219) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with a 1:20 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C219_Purified_C219_Antibody_IHC_032519_updated.png
    IHC staining of purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3antibody (clone C219) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with a 1:20 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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903701 1 ml 515 CHF
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Description

Clone C219 recognizes an internal, highly conserved amino acid sequence (VQEALD & VQAALD) found in both MDR1 and MDR3 isoforms of P-glycoprotein. The specific binding of the clone C219 was stronger in epitope sequence VQAALD (N-Terminal fragment) than the epitope sequence VQEALD (C-terminal fragmant) (Georges E., et al., 1990). Analysis of p-glycoprotein expression in skeletal and cardiac muscle revealed that C219 cross reacts with a 200kDa protein that migrates in the same position as myosin. C219 has also been reported to detect the 185kDa c-erbB2 protein (Liu, et al., 1997). Care should be taken in the interpretation of true positive P-glycoprotein expression when using myosin-containing tissues or tissues known to have p185 c-erbB2 expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Dog, Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was made against SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
IHC-F, ICC, FC, WB, ELISA - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:20 - 1:40 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hisano Y, et al. 2011. J. Biol. Chem. 286: 1758. (WB)
  2. Beck WT, et al. 1996. Cancer Res. 56:3010. (IHC-P, IHC-F, ICC, FC) PubMed
  3. Evans CH, Baker PD. 1992. Cancer Res. 52: 5893. (WB, FC)
  4. Toth K, et al. 1992. Am J Pathol. 140:1009. (IHC-P, ICC, WB)
  5. Finstad CL, et al. 1991. J Histochem Cytochem. 39:1603. (FC, WB, IHC-P
  6. Georges E, et al. 1990. PNAS. 87:152. (ELISA, IHC-P)
  7. Duan Z, et al. 2014. BMC Cancer. 14:681. (WB)
  8. Inui N, et al. 2012. J Gerontol A Biol Sci Med Sci. 67:1049. (FC, IF)
  9. Erickson MA, et al. 2012. J Neuroinflammation. 9:150. (WB)
  10. Rao VV, et al. 1994. Cancer Res. 54:1536. (WB) PubMed
  11. Lee JJ, et al. 1996. Cancer. 77:1892. (IHC) PubMed
Product Citations
  1. Beck W, et al. 1996. Cancer Res. 56:3010-3020. PubMed
  2. Lee J, et al. 1996. Cancer. 77:1892-1898. PubMed
  3. Sophie Kang S, et al. 2016. PLoS One. 11:e0165638. PubMed
  4. Afrouzian M, et al. 2018. Biochem Pharmacol. 156:467. PubMed
Publication Library
RRID
AB_2565033 (BioLegend Cat. No. 903701)

Antigen Details

Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
5244 View all products for this Gene ID
UniProt
View information about CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3) on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 03.25.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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