Purified anti-6-His Epitope Tag Antibody (Previously Covance catalog# MMS-156P)

Pricing & Availability
Clone
6-His (See other available formats)
Regulatory Status
RUO
Other Names
polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Previously
Covance Catalog# MMS-156P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_6-His_Purified_6-His_Antibody_WB_021715
Western Blot showing specificity of 6-His Monoclonal Antibody, Purified, 1:900. Lane 1: MW Marker, Lane 2: PosiTag lysate, Lane 3: Hela cell lysate negative control.
  • 1_6-His_Purified_6-His_Antibody_WB_021715
    Western Blot showing specificity of 6-His Monoclonal Antibody, Purified, 1:900. Lane 1: MW Marker, Lane 2: PosiTag lysate, Lane 3: Hela cell lysate negative control.
  • 2_6-His_Purified_6-His_Antibody_ICC_011421
    Hela cells transfected with multiple tag plasmid were fixed with cold methanol at -20°C for 15 minutes and blocked with 5% FBS for 60 minutes. The cells were then intracellularly stained with either (A) 2 µg/mL anti-mouse IgG1, κ or (B) 2 µg/mL anti-6-His antibody (clone 6-His), or (C) 0.5 µg/mL anti-6-His antibody (clone 6-His), or (D) 0.2 µg/mL anti-6-His antibody (clone 6-His), and incubated overnight at 4°C followed by 2µg/ml (1:250) Alexa Fluor® 594 (Red) goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (Blue). The image was captured with a 60X objective. Exposure times: (A) 1/25, (B) 1/80, (C) 1/80, (D) 1/80.
Cat # Size Price Quantity Avail. Save
906113 25 µL 75,00 CHF
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906101 200 µL 303,00 CHF
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906102 500 µL 474,00 CHF
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Description

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
6xHis Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
Store between 2°C and 8 °C.
Application

WB - Quality tested
ICC, IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000 dilution. For immunocytochemistry, the suggested use is 1:1000 – 1:4000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.

Application References

(PubMed link indicates BioLegend citation)
  1. Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB)
  2. Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (IF) PubMed
  3. Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB)
  4. Bartsch D, et al. 1998. Cell. 95:211. (IF)
  5. Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
  6. Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed
  7. Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
Product Citations
  1. Marques PE et al. 2019. Dev Cell. 50(3):283-295 . PubMed
  2. Mukherjee S et al. 2019. Developmental cell. 49(1):63-76 . PubMed
  3. Huang L, et al. 2013. Nat Biotechnol. 31:350-356. PubMed
  4. Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed
  5. Montegna E, et al. 2012. PLoS One. 7:e31156. PubMed
  6. Fuentes E, et al. 2003. J Biol Chem. 278:21188-21196. PubMed
  7. Dotiwala F, et al. 2016. Nat Med. 22:210-216. PubMed
RRID
AB_2734683 (BioLegend Cat. No. 906113)
AB_2565061 (BioLegend Cat. No. 906101)
AB_2565062 (BioLegend Cat. No. 906102)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about 6-His on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 03.12.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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