PerCP/Cyanine5.5 anti-mouse TCR β chain Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Affinity purified TCR from mouse DO-11.10 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain¹². The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation², in vitro stimulation^2,3, in vivo depletion^4-6, and immunohistochemical staining of acetone-fixed frozen sections^7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)

1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.

Product Citations

1. Oh–Hora M, et al. 2019. J Immunol. 202:2616. PubMed
2. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
3. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
4. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
5. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
6. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
7. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
8. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
9. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
10. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
11. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
12. Nakamoto N, et al. 2019. Nat Microbiol. 4:492. PubMed
13. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
14. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
15. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
16. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
17. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
18. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
19. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
20. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
21. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
22. Anderson CK et al. 2019. Cell Rep. 27(2):537-548 . PubMed
23. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
24. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
25. Kunis G, et al. 2013. Brain. 136:3427. PubMed
26. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
27. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
28. Tong Q, et al. 2022. FEBS Open Bio. 12:1542. PubMed
29. Hutter K, et al. 2020. FEBS J. . PubMed
30. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
31. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
32. Krollmann C, et al. 2022. STAR Protoc. 3:101309. PubMed
33. , et al. 2021. Eur J Immunol. 51:2708. PubMed
34. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
35. Dodard G, et al. 2020. Cell Reports. 32(1):107855.. PubMed
36. Gurusamy M, et al. 2021. Nat Commun. 12:6798. PubMed
37. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
38. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
39. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
40. Kim M, et al. 2018. Immunity. 49:151. PubMed
41. Shissler SC, et al. 2020. Sci Rep. 10:8218. PubMed
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43. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
44. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
45. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
46. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
47. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
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50. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
51. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
52. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
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RRID

AB_1575176 (BioLegend Cat. No. 109227)
AB_1575173 (BioLegend Cat. No. 109228)

Antigen Details

Structure

Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit

Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function

Antigen recognition, T cell activation

Ligand/Receptor

Peptide bound-MHC class I and II

Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID

21577 View all products for this Gene ID

UniProt

View information about TCR beta chain on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

Certificate of Analysis