PerCP/Cyanine5.5 anti-mouse Ly-6G Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Ly-6G transfected EL-4J cell line.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁵. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁵. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4¹⁶.

Additional reported applications (for the relevant formats) include: immunohistochemistry⁹ of frozen sections¹⁰ and paraffin-embedded sections¹¹, depletion^{4, 12-14}, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
2. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
3. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
4. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
5. Cho SM et al. 2018. Journal of neurochemistry. 148(5):625-638 . PubMed
6. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
7. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
8. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
9. Kwon O, et al. 2016. Stem Cell Res. 16: 682-691. PubMed
10. Kim AS, et al. 2021. Cell. 184(17):4414-4429.e19. PubMed
11. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
12. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
13. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
14. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
15. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
16. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
17. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
18. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
19. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
20. Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
21. Kwon O, et al. 2014. Proc Natl Acad Sci U S A. 111:592. PubMed
22. Liu Y, et al. 2015. Sci Rep. 5:17215. PubMed
23. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
24. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
25. Francian A, et al. 2021. J Drug Target. 29:754. PubMed
26. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
27. McCullough LD, et al. 2021. J Clin Invest. 131:. PubMed
28. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
29. Klopfenstein N, et al. 2021. PLoS Pathog. 17:e1009387. PubMed
30. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
31. Lebratti T, et al. 2021. eLife. 10:00. PubMed
32. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
33. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
34. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
35. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
36. Moses K, et al. 2016. J Leukoc Biol. 99: 811 - 823. PubMed
37. Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed
38. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
39. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
40. Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
41. Kovacic B, et al. 2014. Haematologica. 99:1006. PubMed
42. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
43. Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed
44. Zhang T, et al. 2022. Oncogene. . PubMed
45. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
46. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
47. Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed
48. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
49. Dang MT, et al. 2021. Cell Reports. 34(13):108917. PubMed
50. Körner A, et al. 2019. Proc Natl Acad Sci U S A. 116:20623. PubMed
51. Acker KP, et al. 2019. iScience. 19:281. PubMed
52. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
53. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
54. Devaraj A, et al. 2021. J Clin Invest. 131:. PubMed
55. Gurung P, et al. 2016. Proc Natl Acad Sci U S A. 113: 4452-4457. PubMed
56. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
57. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
58. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
59. Dang MT, et al. 2021. STAR Protoc. 2:100957. PubMed
60. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
61. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
62. Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
63. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
64. Mattila JT, et al. 2017. J Immunol. 199:806. PubMed
65. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
66. Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed
67. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
68. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
69. Chen Z, et al. 2016. Nat Commun. 7:11596. PubMed
70. Lokken K, et al. 2014. PLoS Pathog. 10:1004049. PubMed
71. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
72. Sun X, et al. 2020. Cell Metabolism. 31(1):189-206.e8.. PubMed
73. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
74. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
75. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
76. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
77. Karki R, et al. 2020. bioRxiv. . PubMed
78. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
79. Dymek B, et al. 2022. J Inflamm Res. 15:5621. PubMed
80. Milich LM, et al. 2021. J Exp Med. 218:. PubMed
81. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
82. Karki R, et al. 2021. Cell. 184:149. PubMed
83. Jablonski K, et al. 2015. PLoS One. 10: 0145342. PubMed
84. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
85. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
86. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
87. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
88. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
89. Ireland L, et al. 2020. Front Immunol. 11:297. PubMed
90. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
91. Verma M, et al. 2021. J Exp Med. 218:. PubMed
92. Nakayama Y, et al. 2020. Proc Natl Acad Sci U S A. 117:14365. PubMed
93. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
94. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
95. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
96. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
97. Trivedi S, et al. 2020. Elife. 9:00. PubMed
98. Amber Smith, Amanda Smith 2016. Sci Rep. 6:38703. PubMed
99. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
100. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed

RRID

AB_1877272 (BioLegend Cat. No. 127615)
AB_1877271 (BioLegend Cat. No. 127616)

Antigen Details

Structure

A 21-35 kD GPI-anchorded membrane protein

Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type

Granulocytes, Macrophages, Monocytes

Biology Area

Immunology, Innate Immunity

Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID

546644 View all products for this Gene ID

UniProt

View information about Ly-6G on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

Certificate of Analysis