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PerCP/Cyanine5.5 anti-mouse CD73 Antibody

Pricing & Availability
Clone
TY/11.8 (See other available formats)
Regulatory Status
RUO
Other Names
Ecto-5'-nucleotidase
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
TYslash11dot8_PerCPCyanine55_CD73_Antibody_101818
C57BL/6 mouse splenocytes were stained with CD3e APC and CD73 (clone TY/11.8) PerCP/Cyanine5.5 (left), or rat IgG1, ? PerCP/Cyanine5.5 isotype control (right).
  • TYslash11dot8_PerCPCyanine55_CD73_Antibody_101818
    C57BL/6 mouse splenocytes were stained with CD3e APC and CD73 (clone TY/11.8) PerCP/Cyanine5.5 (left), or rat IgG1, ? PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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127213 25 µg 147 CHF
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127214 100 µg 347 CHF
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Description

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO cells transfected with mouse CD73
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
  2. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations
  1. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  2. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  3. Dumas SJ, et al. 2021. STAR Protocols. 2(3):100523. PubMed
  4. Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed
  5. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  6. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  7. Dumas SJ, et al. 2020. J Am Soc Nephrol. 31:118. PubMed
  8. Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
  9. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  10. Raso F, et al. 2018. J Clin Invest. 128:4163. PubMed
  11. Young JS, et al. 2018. Front Immunol. 9:1385. PubMed
  12. Ott M, et al. 2020. JCI Insight. 5(17):134386. PubMed
RRID
AB_11219608 (BioLegend Cat. No. 127213)
AB_11219403 (BioLegend Cat. No. 127214)

Antigen Details

Structure
69 kD GPI-anchored surface protein
Distribution

Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73

Function
A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor
Nucleoside 5' monophosphate
Cell Type
B cells, Mesenchymal Stem Cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.

Regulation
Expression is regulated by the development and maturation of leukocytes
Gene ID
23959 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 10.18.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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