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PerCP/Cyanine5.5 anti-mouse CD194 (CCR4) Antibody

Pricing & Availability
Clone
2G12 (See other available formats)
Regulatory Status
RUO
Other Names
CC CKR-4, CHEMR1, Cmkbr4, LESTR, MGC151418, Sdf1r
Isotype
Armenian Hamster IgG
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Product Citations
publications
2G12_PerCpCyanine55_CD194_1_Antibody_102523
Hyper-immunized BALB/c splenocytes stained with CD4 (clone GK1.5) APC and CD194 (clone 2G12) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG PerCP/Cyanine5.5 Isotype Control (right).
  • 2G12_PerCpCyanine55_CD194_1_Antibody_102523
    Hyper-immunized BALB/c splenocytes stained with CD4 (clone GK1.5) APC and CD194 (clone 2G12) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG PerCP/Cyanine5.5 Isotype Control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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131219 25 µg 176 CHF
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131220 100 µg 382 CHF
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Description

Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85% identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naïve T cells, bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes and express cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity to migrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play an important role in the ligand-mediated recruitment of T cells into the skin in mice and humans, specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site of injury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucial for the induction and maintenance of self-tolerance and are present in peripheral tissues such as skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor and its ligand CCL22 (5).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Saito K, et al. 2008. J. Immunol. 181:6889. PubMed
  2. Ueha S, et al. 2007. J. Leukoc. Biol. 82:1230. PubMed
  3. 3. Sharma R, et al. 2009 J. Immunol. 183:1065 (FC) PubMed
  4. Dogan R, et al. 2011. J. Leukoc. Biol. 89:93. PubMed
  5. Liu H, et al. 2011. Sci Transl Med. 3:82. PubMed.
  6. Shankar SP, et al. 2012. J Immunol. 188:6347. PubMed.
RRID
AB_2750070 (BioLegend Cat. No. 131219)
AB_2750071 (BioLegend Cat. No. 131220)

Antigen Details

Structure
GPCR, chemokine receptor, seven transmembrane receptor
Distribution

Memory T cells, macrophages, platelets, basophils, Th2 cells, and Tregs

Function
CCR4 and its ligands (CCL17 and CCL22) are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
Interaction
Interaction between CCR4 and its ligand TARC/CCL17 on activated endothelial cells mediates T cell extravasation by stimulating integrin-dependent adhesion
Ligand/Receptor
CCl17 and CCL22
Cell Type
Basophils, Embryonic Stem Cells, Macrophages, Platelets, T cells, Th2, Tregs
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Biederman T, et al. 2002. Eur. J. Immun. 32:3171.
2. Mirshahpanah P, et al. 2008. Exp. Dermatol. 17:30.
3. Kusumoto M, et al. 2007. J. Interferon Cytokine Res 27:901.
4. Clark RA and Kupper TS. 2006. Blood 109:194.
5. Lee I, et al. 2005. J. Exp. Med. 201:1037.

Gene ID
12773 View all products for this Gene ID
UniProt
View information about CD194 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 07.05.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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