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PerCP/Cyanine5.5 anti-human IgD Antibody

Pricing & Availability
Clone
W18340F (See other available formats)
Regulatory Status
RUO
Other Names
Ig delta chain C region
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18340F_PerCPCy7_IgD_Antibody_070721.png
Human peripheral blood lymphocytes were stained with CD19 APC and anti-human IgD (clone W18340F) PerCP/Cyanine5.5 (left) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • W18340F_PerCPCy7_IgD_Antibody_070721.png
    Human peripheral blood lymphocytes were stained with CD19 APC and anti-human IgD (clone W18340F) PerCP/Cyanine5.5 (left) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
307809 25 tests 171 CHF
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307810 100 tests 375 CHF
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Description

IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell. (BioLegend Website)

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Native human IgD protein (full length)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
Application Notes

This clone does not cross react with mouse tissues.

RRID
AB_2894457 (BioLegend Cat. No. 307809)
AB_2894457 (BioLegend Cat. No. 307810)

Antigen Details

Distribution

Naïve B cells

Function
Antigen binding, B cell activation
Cell Type
B cells
Biology Area
Immunology
Antigen References
  1. Geisberger R, et al. 2006. Immunology. 118:429-37.
  2. Weller S, et al. 2005. Eur J Immunol. 35:2789-92.
  3. Brandtzaeg P & Johansen FE. 2005. Immunol Rev. 206:32-63.
Gene ID
3495 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 07.07.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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