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PerCP/Cyanine5.5 anti-human CD71 Antibody

Pricing & Availability
Clone
CY1G4 (See other available formats)
Regulatory Status
RUO
Workshop
A015
Other Names
T9, Transferrin receptor
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
CY1G4_PerCPCyanine55_CD71_Antibody_080918
PHA-stimulated (day 3) human peripheral blood lymphocytes were stained with CD71 (clone CY1G4) PerCP/Cyanine5.5 (filled histogram), or mouse IgG2a, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • CY1G4_PerCPCyanine55_CD71_Antibody_080918
    PHA-stimulated (day 3) human peripheral blood lymphocytes were stained with CD71 (clone CY1G4) PerCP/Cyanine5.5 (filled histogram), or mouse IgG2a, ? PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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334113 25 tests 176 CHF
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334114 100 tests 382 CHF
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Description

CD71 is a 95 kD type II homodimeric transmembrane glycoprotein also known as T9 and transferrin receptor. It is expressed on proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NALM-6 pre-B cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  2. Cho A, et al. 2021. Nature. Online ahead of print. PubMed
  3. Pellin D, et al. 2019. Nat Commun. 10:2395. PubMed
  4. Herland A, et al. 2020. Nat Biomed Eng. 421:4. PubMed
  5. Chou DB, et al. 2020. Nat Biomed Eng. 0.440277778. PubMed
  6. Cho A, et al. 2022. J Exp Med. 219:. PubMed
RRID
AB_2563174 (BioLegend Cat. No. 334113)
AB_2563175 (BioLegend Cat. No. 334114)

Antigen Details

Structure
Transferrin receptor family, type II glycoprotein, 95 kD
Distribution

Proliferating cells, reticulocytes, erythroid precursors

Function
Mediates uptake of iron
Ligand/Receptor
Transferrin
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Hentze M, et al. 1996. P. Natl. Acad. Sci. USA 93:8175.
2. Trowbridge I, et al. 1993. Annu. Rev. Cell Biol. 9:129.

Gene ID
7037 View all products for this Gene ID
UniProt
View information about CD71 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08.09.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • APC/Fire™ 750 anti-human CD71

  • PE/Cyanine7 anti-human CD71

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  • PE/Cyanine5 anti-human CD71
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