PerCP/Cyanine5.5 anti-human CD196 (CCR6) Antibody

Pricing & Availability
G034E3 (See other available formats)
Regulatory Status
Other Names
Mouse IgG2b, κ
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Product Citations
Human peripheral lymphocytes were stained with CD19 APC and CD196 (CCR6, clone G034E3) PerCP/Cyanine5.5 (left), or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
  • G034E3_PerCPCyanine55_CD196_Antibody_110118
    Human peripheral lymphocytes were stained with CD19 APC and CD196 (CCR6, clone G034E3) PerCP/Cyanine5.5 (left), or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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353405 25 tests 176 CHF
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353406 100 tests 382 CHF
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CCR6, also known as CD196, is a chemokine receptor that is expressed on immature dendritic cells, B lymphocytes, and memory T cells. CCR6 binds CCL20, although members of the β defensin family also bind CCR6 with a lower affinity. CCR6 positive cells, and its ligand CCL20, have been detected in numerous organs, especially the secondary lymphoid organ. CCL20 is selectively made by the follicle-associated epithelium (FAE) overlying Peyers Patches (PPs) and isolated lymphoid follicles (ILFs). CCL20 contributes to the recruitment of CCR6-expressing B cells to these structures. In humans, CCR6 can function to mediate arrest of T cells on dermal endothelial cells and is highly expressed on T cells resident in both normal and psoriatic skin. CCR6 and/or CCL20 have been implicated in the pathogenesis of rheumatoid arthritis and inflammatory bowel disease. Human T cells that are able to produce IL-17 express CCR6. It suggests that CCL20 and CCR6 have a role in inflammatory diseases by recruiting Th17 cells to target tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Host Species
CCR6-transfected cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Product Citations
  1. Balachandran H, et al. 2022. Cell Rep. 38:110345. PubMed
  2. Matsui K, et al. 2015. PLoS One. 10: 0137195. PubMed
  3. Andrews SF et al. 2019. Immunity. 51(2):398-410 . PubMed
  4. Brito-Pérez Y, et al. 2021. Immun Inflamm Dis. 9:1541. PubMed
  5. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  6. Zhao L, et al. 2016. Rheumatology. 10.1093/rheumatology/kew360. PubMed
  7. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  8. Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed
AB_10918985 (BioLegend Cat. No. 353405)
AB_10918437 (BioLegend Cat. No. 353406)

Antigen Details

Chemokine receptor, GPCR, seven transmembrane receptor

Immature dendritic cells, B lymphocytes, memory T cells, Th17 cells

Host defense, important for recruitment of B cells to secondary lymphoid organs, mediates arrest of T cells on dermal endothelial cells
CCL20 (MIP-3α)
Cell Type
B cells, Dendritic cells, T cells, Th17
Biology Area
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Zaballos A, et al. 1996. Biochem. Bioph. Res. Co. 227:846.
2. Yang D, et al. 1999. Science 286:525.
3. MacDonald KG, et al. 2007. Am. J. Pathol. 170:1229.
4. Homey B, et al. 2000. J. Immunol. 164:6621.
5. Hirota K, et al. 2007. J. Exp. Med. 204:2803.
6. Singh SP, et al. 2008. J. Immunol. 180:214.

Gene ID
1235 View all products for this Gene ID
View information about CD196 on

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 11.01.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD196 (CCR6)

  • Alexa Fluor® 647 anti-human CD196 (CCR6)

  • Brilliant Violet 421™ anti-human CD196 (CCR6)

  • PerCP/Cyanine5.5 anti-human CD196 (CCR6)

  • PE anti-human CD196 (CCR6)

  • FITC anti-human CD196 (CCR6)

  • Alexa Fluor® 488 anti-human CD196 (CCR6)

  • APC anti-human CD196 (CCR6)

  • PE/Cyanine7 anti-human CD196 (CCR6)

  • Brilliant Violet 605™ anti-human CD196 (CCR6)

  • Brilliant Violet 785™ anti-human CD196 (CCR6)

  • Brilliant Violet 510™ anti-human CD196 (CCR6)

  • Brilliant Violet 650™ anti-human CD196 (CCR6)

  • Purified anti-human CD196 (CCR6) (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human CD196 (CCR6)

  • APC/Cyanine7 anti-human CD196 (CCR6)

  • Alexa Fluor® 700 anti-human CD196 (CCR6)

  • Brilliant Violet 711™ anti-human CD196 (CCR6)

  • TotalSeq™-A0143 anti-human CD196 (CCR6)

  • Pacific Blue™ anti-human CD196 (CCR6)

  • TotalSeq™-C0143 anti-human CD196 (CCR6)

  • APC/Fire™ 750 anti-human CD196 (CCR6)

  • TotalSeq™-B0143 anti-human CD196 (CCR6)

  • TotalSeq™-D0143 anti-human CD196 (CCR6)

  • PE/Fire™ 640 anti-human CD196 (CCR6)


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