PerCP anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Pricing & Availability
Clone
RB6-8C5 (See other available formats)
Regulatory Status
RUO
Other Names
Gr-1
Isotype
Rat IgG2b, κ
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Product Citations
publications
RB6-8C5_PerCP_021808
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) PerCP (filled histogram) or rat IgG2b, κ PerCP isotype control (open histogram).
  • RB6-8C5_PerCP_021808
    C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) PerCP (filled histogram) or rat IgG2b, κ PerCP isotype control (open histogram).
Compare all formats See PerCP spectral data
Cat # Size Price Quantity Check Availability Save
108425 25 µg 99 CHF
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108426 100 µg 270 CHF
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Description

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Stubelius A, et al. 2017. Immunobiology. 10.1016/j.imbio.2017.05.007. PubMed
  2. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  3. Cossio-Ayala M, et al. 2017. Peptides. 10.1016/j.peptides.2017.06.006. PubMed
  4. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  5. Man SM et al. 2016. Cell. 167(2):382-396 . PubMed
  6. Chen PY, et al. 2019. Nat Metab. 0.675. PubMed
  7. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  8. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  9. Siegfried A, et al. 2013. J Immunol. 191:3913. PubMed
  10. Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed
  11. Steinmetz O, et al. 2016. J Infect Dis. 10.1093/infdis/jiv763. PubMed
  12. SL C, et al. 2016. JCI Insight. 1: 83116. PubMed
  13. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  14. Karki R, et al. 2020. JCI Insight. 5:00. PubMed
  15. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
RRID
AB_893560 (BioLegend Cat. No. 108425)
AB_893557 (BioLegend Cat. No. 108426)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All Ly-6G/Ly-6C Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC, IHC, IP, WB
FITC anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PE anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PE/Cyanine5 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Purified anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC, IHC, IP, WB
PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Alexa Fluor® 488 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC, IHC
Alexa Fluor® 647 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 711™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PerCP anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 570™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC, IP, CMCD, Depletion, IHC, WB
Brilliant Violet 510™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 605™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 650™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Alexa Fluor® 594 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 IHC-F, FC
Purified anti-mouse Ly-6G/Ly-6C (Gr-1) (Maxpar® Ready) RB6-8C5 FC, CyTOF®
PE/Dazzle™ 594 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Fire™ 750 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
TotalSeq™-A0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
TotalSeq™-C0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
TotalSeq™-B0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
Spark Blue™ 550 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Fire™ 810 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Spark Violet™ 423 anti-mouse Ly-6G/Ly-6C (GR-1) Antibody RB6-8C5 FC
Spark UV™ 387 anti-mouse Ly-6G/Ly-6C (GR-1) RB6-8C5 FC
Go To Top Version: 2    Revision Date: 12.09.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Biotin anti-mouse Ly-6G/Ly-6C (Gr-1)

  • FITC anti-mouse Ly-6G/Ly-6C (Gr-1)

  • PE anti-mouse Ly-6G/Ly-6C (Gr-1)

  • PE/Cyanine5 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Purified anti-mouse Ly-6G/Ly-6C (Gr-1)

  • PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Alexa Fluor® 488 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Alexa Fluor® 647 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Brilliant Violet 711™ anti-mouse Ly-6G/Ly-6C (Gr-1)

  • APC/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1)

  • PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • PerCP anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Brilliant Violet 570™ anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Ultra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Brilliant Violet 510™ anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Brilliant Violet 605™ anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Brilliant Violet 650™ anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Alexa Fluor® 594 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Purified anti-mouse Ly-6G/Ly-6C (Gr-1) (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • APC/Fire™ 750 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • TotalSeq™-A0116 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • TotalSeq™-C0116 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • TotalSeq™-B0116 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Spark Blue™ 550 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • APC/Fire™ 810 anti-mouse Ly-6G/Ly-6C (Gr-1)

  • Spark Violet™ 423 anti-mouse Ly-6G/Ly-6C (GR-1) Antibody

  • Spark UV™ 387 anti-mouse Ly-6G/Ly-6C (GR-1)

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