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PE/Dazzle™ 594 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Phycoerythrin/Dazzle™ 594, SAv-PE/Dazzle™ 594
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Product Citations
publications
See PE/Dazzle™ 594 spectral data
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405247 25 µg 112 CHF
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405248 100 µg 300 CHF
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Description

Streptavidin binds to biotin with high affinity. Streptavidin - PE/Dazzle™ 594 is useful for detecting biotinylated antibodies. The excitation of PE/Dazzle™ 594 by the 488 nm laser light induces a light emission maximum of 610 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The Streptavidin-PE/Dazzle™ 594 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of Streptavidin-PE/Dazzle™ 594 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE/ Dazzle™ 594 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤0.06 µg per million cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin-PE/Dazzle™ 594 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Dazzle™ 594 is 540 kD and Streptavidin alone is 52 kD.

Product Citations
  1. Kurane T, et al. 2020. Okajimas Folia Anat Jpn. 96:49:00. PubMed
  2. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  3. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  4. Reed J, et al. 2020. Bio Protoc. 10:e3607. PubMed
  5. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  6. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  7. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  8. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed

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Go To Top Version: 3    Revision Date: 06.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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