PE/Cyanine7 anti-mouse TCR β chain Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Affinity purified TCR from mouse DO-11.10 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain¹². The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation², in vitro stimulation^2,3, in vivo depletion^4-6, and immunohistochemical staining of acetone-fixed frozen sections^7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)

1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.

Product Citations

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5. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
6. Martens R, et al. 2020. Nat Commun. 11:1114. PubMed
7. Failer T, et al. 2022. J Clin Invest. . PubMed
8. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
9. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
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15. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
16. Gordon RA, et al. 2020. PLoS One. 15:e0226396. PubMed
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18. Krementsov DN, et al. 2018. Front Immunol. 9:1622. PubMed
19. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
20. Ribeiro-Gomes F, et al. 2015. Cell Death Dis. 6: 2018. PubMed
21. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
22. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
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24. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
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26. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
27. Sun H, et al. 2022. Front Immunol. 13:818017. PubMed
28. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
29. Sadik A, et al. 2020. Cell. 1252:182. PubMed
30. Kyburz A, et al. 2017. Clin Exp Allergy. 47:1331. PubMed
31. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
32. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
33. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
34. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
35. Sencio V, et al. 2020. Cell Reports. 30(9):2934-2947. PubMed
36. Udden SN et al. 2019. eLife. 8 pii: e40396. PubMed
37. Matas-Rico E, et al. 2021. Cell Rep. 37:110013. PubMed
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41. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
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45. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
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RRID

AB_893627 (BioLegend Cat. No. 109221)
AB_893625 (BioLegend Cat. No. 109222)

Antigen Details

Structure

Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit

Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function

Antigen recognition, T cell activation

Ligand/Receptor

Peptide bound-MHC class I and II

Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID

21577 View all products for this Gene ID

UniProt

View information about TCR beta chain on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis