PE anti-Notch 1 Antibody

Pricing & Availability
Clone
mN1A (See other available formats)
Other Names
Neurogenic locus notch homolog protein 1, Notch-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
Notch-1_PE_051707
Jurkat cells Intracellularly stained with Notch-1 PE
  • Notch-1_PE_051707
    Jurkat cells Intracellularly stained with Notch-1 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
629105 25 µg 85 CHF
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629106 100 µg 170 CHF
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Description

Notch1, also known as neurogenic locus notch homolog protein 1, is a large >270 kD protein that functions as a receptor for the membrane ligands Jagged1, Jagged2 and Delta1 to regulate cell fate decisions. Upon ligand activation, the transmembrane Notch1 receptor is cleaved by TNF-alpha converting enzyme (TACE) to produce a membrane-associated intermediate fragment (NEXT). This fragment is further cleaved by presenilin-dependent gamma secretase to release a notch-derived peptide containing the intracellular fragment from the membrane. The released Notch intracellular fragment (NICD) translocates to the nucleus and forms a transcriptional complex with the RBP-J κ transcriptional activator complex to alter differentiation, proliferation, and apoptotic programs. Notch 1 is highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells) with lower levels of expression observed in the spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart. The transmembrane Notch protein is a heterodimeric protein consisting of a C-terminal fragment and N-terminal fragment (probably linked by disulphide bonds) containing 5 ankyrin repeats, 36 EGF repeats, and 3 Notch/Lin repeats. Notch1 can be modified by phosphorylation. The mN1A monoclonal antibody reacts with the intracellular domain of mouse and human Notch1 and has been reported to have highest affinity for activated intracellular Notch1 and lower affinity for full-length unprocessed/heterodimeric Notch1 forms. This antibody does not recognize rat Notch1 or cross-react with Notch2, 3, or 4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Notch1 GST fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications of this antibody (for the relevant formats) include: Western blotting1,2,3, and immunohistochemistry4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Huppert SS, et al. 2000. Nature 405:966. (WB)
  2. Ray WJ, et al. 1999. Proc. Natl. Acad. Sci. USA 96:3263. (WB)
  3. DeStrooper B, et al. 1999. Nature 398:518. (WB)
  4. Sun H, et al. 2007. J. Cell Biol. 177:647. (IHC) PubMed
  5. Jundt F, et al. 2008. Leukemia. 22:1587. (IHC) PubMed
  6. Liu JC, et al. 2012. PNAS 109:5832. PubMed.
Product Citations
  1. Kongkavitoon P, et al. 2016. PLoS One. 11: 0146696. PubMed
  2. Britton GJ, et al. 2017. Elife. 6:. PubMed
Publication Library
RRID
AB_2282905 (BioLegend Cat. No. 629105)
AB_2251468 (BioLegend Cat. No. 629106)

Antigen Details

Structure
Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 36 EGF repeats, 3 Notch/Lin repeats. Predicted molecular weight 271 kD.
Distribution
Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart.
Function
Receptor for the membrane ligands Jagged1, Jagged2 and Delta1 to regulate cell fate decisions.
Modification
Phosphorylation
Cell Type
T cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

1. Huppert SS, et al. 2000. Nature 405:966.
2. Kopan R, et al. 1993. J. Cell Biol. 121:631.
3. Saxena MT, et al. 2001. J. Biol. Chem. 276:40268.
4. Mizutani T, et al. 2001. Proc. Natl. Acad. Sci. USA 98:9026.

Gene ID
4851 View all products for this Gene ID
UniProt
View information about Notch1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 11.18.2013

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