PE anti-mouse Notch 4 Antibody

Pricing & Availability
Clone
HMN4-14 (See other available formats)
Other Names
Proto-oncogene of int-3
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMN4-14_PE_081908.jpg
FN4/CHO (mouse Notch-4 transfected) cells stained with HMN4-14 PE
  • HMN4-14_PE_081908.jpg
    FN4/CHO (mouse Notch-4 transfected) cells stained with HMN4-14 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
128407 50 µg 150 CHF
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Description

The Notch receptors and their ligands are highly conserved from invertebrates to mammals.The Delta-like 1 (Dll1) is one of the four or five Notch ligands identified.The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus.This translocation triggers a series of signaling process.Delta-like 1 is reported to be essential for the maintenance of marginal zone B cells in normal mice and engagement of Notch 1 by Dll 1 promotes differentiation of B lymphocytes to antibody-secreting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Notch4-Fc recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Moriyama Y, et al.2008. Intl J. Immunol. 20:763.
  2. Reilla LV, et al. 2011. J. Immunol. 187:4629. PubMed.
Product Citations
  1. Yan Q, et al. 2010. Am J Pathol. 176:2921. PubMed
  2. Chennupati V, et al. 2016. J Immunol. 197: 771 - 782. PubMed
RRID
AB_1133997 (BioLegend Cat. No. 128407)

Antigen Details

Structure
Type 1 transmembrane receptor with a large extracellular domain consisting of 29 -36 EGF( epidermal growth factor) and three LNR (Lin12-Notch) repeats.
Distribution

Notch 4 is preferentially expressed in endothelial cells.

Function
Regulation of cell development, including myogenesis, neurogenesis, and lymphocyte development. Notch 4 seems to play a crucial role in vasculogenesis and angiogenesis.
Ligand/Receptor
Jagged 1, Jagged2, Delta-like 1, Delta-like3 and Delta-like 4
Cell Type
Endothelial cells
Biology Area
Immunology
Antigen References

1.Ehebauer MT,et al. 2006. Biochem J.392:13.
2.Shimizu K,et al. 2000.Mol Cell Biology. 20:18
3. Tanigaki K,et al.2007. Nature immunol. 8:451.

Gene ID
18132 View all products for this Gene ID
UniProt
View information about Notch 4 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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