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PE anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
D7_PE_091207
C57BL/6 mouse splenocytes stained with D7 PE
  • D7_PE_091207
    C57BL/6 mouse splenocytes stained with D7 PE
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108107 50 µg 122 CHF
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108108 200 µg 293 CHF
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Perveen S, et al. 2012. PLoS One. 7:e51945. PubMed
  2. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  3. Enquist I, et al. 2009. Stem Cells. 27:744. PubMed
  4. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  5. Wang L, et al. 2020. Cell Metab. 32:860. PubMed
  6. Yang M, et al. 2020. Cell Prolif. 53:e12784. PubMed
  7. Sieveking D, et al. 2010. J Exp Med. 207:345. PubMed
  8. Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
  9. Li J, et al. 2022. Int J Biol Sci. 18:2091. PubMed
  10. Cui Z, et al. 2022. Bone Res. 10:58. PubMed
  11. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  12. Li J, et al. 2019. JCI Insight. 5. PubMed
  13. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  14. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  15. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  16. Phan QM, et al. 2020. Elife. 9:00. PubMed
  17. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  18. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  19. Zhao J, et al. 2018. Stem Cell Reports. 10:180. PubMed
  20. Marinescu CI, et al. 2021. Stem Cell Res Ther. 12:319. PubMed
  21. Hu Y, et al. 2021. Bioact Mater. 2905:6. PubMed
  22. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
  23. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  24. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  25. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  26. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  27. N. H, et al. 2017. Journal of Vascular Research. 10.1159/000464287. PubMed
  28. Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed
  29. Li CJ, et al. 2018. J Clin Invest. 128:5251. PubMed
  30. Hou J, et al. 2017. Mol Med Rep. 16:1502. PubMed
  31. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  32. Xu X, et al. 2022. EMBO Rep. 23:e53509. PubMed
  33. Zelco A, et al. 2020. Front Cell Neurosci. 0.75625. PubMed
  34. Schwörer S, et al. 2016. Nature. 540:428-432. PubMed
  35. Rüder C, et al. 2014. PLoS One. 9:104644. PubMed
  36. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  37. Kwiecien K, et al. 2020. Sci Rep. 10:13702. PubMed
  38. Bruno C, et al. 2020. Brain Commun. 2:fcaa133. PubMed
  39. Miura Y et al. 2018. eLife. 7 pii: e36572. PubMed
  40. Ge Q, et al. 2018. Mol Med Rep. 17:1667. PubMed
  41. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  42. Kopinke D et al. 2017. Cell. 170(2):340-351 . PubMed
  43. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  44. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  45. Young K, et al. 2021. Cell Stem Cell. . PubMed
RRID
AB_313344 (BioLegend Cat. No. 108107)
AB_313345 (BioLegend Cat. No. 108108)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 07.11.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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