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PE anti-mouse IL-1α Antibody

Pricing & Availability
Clone
ALF-161 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)
Isotype
Armenian Hamster IgG
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Product Citations
publications
ALF-161_PE_IL-1a_Antibody_080819
BALB/c macrophages were stimulated with LPS (3 days) and stained with CD11b APC and IL-1α (clone ALF-161) PE (left) or Armenian Hamster IgG PE isotype control (right).
  • ALF-161_PE_IL-1a_Antibody_080819
    BALB/c macrophages were stimulated with LPS (3 days) and stained with CD11b APC and IL-1α (clone ALF-161) PE (left) or Armenian Hamster IgG PE isotype control (right).
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Cat # Size Price Quantity Check Availability Save
503203 25 µg 110 CHF
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The ALF-161 antibody reacts with precursor, secreted and membrane-associated forms of mouse IL-1α. The ALF-161 antibody can neutralize the bioactivity of natural or recombinant IL-1α.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli - expressed, recombinant mouse IL-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Capture: The purified ALF-161 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5034 antibody (Cat. No. 503401) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
ELISA Capture: The purified ALF-161 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated BL1a-89 antibody (Cat. Nos. 512503 & 512504) as the detection antibody and recombinant mouse IL-1a (Cat. No. 580309) as the standard.
Neutralization: Clone ALF-161 has been described to have IL-1a neutralizing properties based on the in vivo mice model3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended (Cat. No. 503207-503212).

Application References

(PubMed link indicates BioLegend citation)
  1. Fuhlbrigge R, et al. 1988. J. Immunol. 141:2643.
  2. Rogers HW, et al. 1992. P. Natl. Acad.Sci. USA 89:1011.
  3. Yazdi AS, et al. 2010. P. Natl. Acad. Sci. USA 107:19449. (Neut)
Product Citations
  1. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  2. Cullis J, et al. 2017. Cancer Immunol Res. 5:182. PubMed
  3. Wei H, et al. 2021. Malar J. 20:89. PubMed
  4. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
  5. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
  6. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
  7. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
RRID
AB_315281 (BioLegend Cat. No. 503203)

Antigen Details

Structure
Cytokine; 17.4 kD (Mammalian)
Bioactivity
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
Cell Targets
B cells, T cells, monocytes, fibroblasts
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Regulation
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID
16175 View all products for this Gene ID
UniProt
View information about IL-1alpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All IL-1α Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-1α ALF-161 ICFC
Purified anti-mouse IL-1α ALF-161 ELISA Capture,IP,WB
Ultra-LEAF™ Purified anti-mouse IL-1α ALF-161 ELISA Capture,ELISPOT Capture,ICFC,IP,Neut,WB
Go To Top Version: 2    Revision Date: 08.09.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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