PE anti-mouse/human Helios Recombinant Antibody

Pricing & Availability
Clone
QA20A52 (See other available formats)
Regulatory Status
RUO
Other Names
IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
QA20A52_PE_Helios_RECOM_Antibody_091621.png
C57BL/6 mouse splenocytes were surface stained with anti-mouse CD4 APC and then fixed and perm using True-Nuclear™ Transcription Factor Buffer Set. Cells were then intracellularly stained with anti-mouse/human Helios recombinant (clone QA20A52) PE (left) or rat IgG1, κ isotype control (right).
  • QA20A52_PE_Helios_RECOM_Antibody_091621.png
    C57BL/6 mouse splenocytes were surface stained with anti-mouse CD4 APC and then fixed and perm using True-Nuclear™ Transcription Factor Buffer Set. Cells were then intracellularly stained with anti-mouse/human Helios recombinant (clone QA20A52) PE (left) or rat IgG1, κ isotype control (right).
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105403 25 tests 175,00 CHF
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105404 100 tests 345,00 CHF
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Description

Helios is a member of the Ikaros family of zinc finger transcription factors. It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members. Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes. It is found at high levels in a subpopulation of regulatory T cells. Helios plays an important role in T cell development and homeostasis. Overexpression of Helios profoundly alters αβ T cell differentiation and activation. It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function. Helios is also involved in tumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA20A52 is able to completely block the staining of clone 22F6 on target cells

RRID
AB_2894413 (BioLegend Cat. No. 105403)
AB_2894413 (BioLegend Cat. No. 105404)

Antigen Details

Structure
A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution

Regulatory T cells (Tregs), conventional T cells, thymocytes.

Function
Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kelly CM, et al. 1998. Curr Biol. 8:508.
2. Dovat S, et al. 2005. J Immunol. 175:3508.
3. Cortes M, et al. 1999. Curr Opin Immunol. 11:167.
4. Cai Q, et al. 2009. J Immunol. 183:2303.
5. Zhang Z, et al. 2007. Blood. 109:2190.
6. Hahm K, et al. 1998. Genes Dev. 12:782.

Gene ID
22779 View all products for this Gene ID 22807 View all products for this Gene ID
UniProt
View information about Helios on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 09.16.2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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