PE anti-mouse Granzyme A Antibody

Pricing & Availability
Clone
3G8.5 (See other available formats)
Other Names
Hf, SE1, TSP1, Ctla3, TSP-1, Ctla-3, AW494114
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
3G8.5_PE_GranzymeA_Antibody_ICFC_1_022415
CD3/CD28 (three days) stimulated BALB/c splenocytes were intracellularly stained with CD8 APC and granzyme A (clone 3G8.5) PE (top) or mouse IgG2b, κ PE isotype control (bottom).
  • 3G8.5_PE_GranzymeA_Antibody_ICFC_1_022415
    CD3/CD28 (three days) stimulated BALB/c splenocytes were intracellularly stained with CD8 APC and granzyme A (clone 3G8.5) PE (top) or mouse IgG2b, κ PE isotype control (bottom).
  • 3G8.5_PE_GranzymeA_Antibody_ICFC_2_022415
See PE spectral data
Cat # Size Price Quantity Avail. Save
149703 25 µg 125 CHF
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149704 100 µg 285 CHF
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Description

Granzyme A is a serine protease that is similar to Granzyme B. However, unlike granzyme B, granzyme A is a homodimer. It is expressed in cytotoxic T cells and natural killer cells (NK), but is not essential for induction of apoptosis or DNA fragmentation. Cell death induced by granzyme A is slower and morphologically different than the cell death induced by granzyme B. It plays a crucial role in infection-mediated inflammation as well as immunological responses against viruses, bacteria, and tumor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant granzyme A of mouse origin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Fehniger TA, et al. 2007. Immunity 26:798. (ICFC)
Product Citations
  1. Uchil PD, et al. 2019. Cell Host Microbe. 25:87. PubMed
  2. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
Publication Library
RRID
AB_2565309 (BioLegend Cat. No. 149703)
AB_2565310 (BioLegend Cat. No. 149704)

Antigen Details

Structure
29 kD, Homodimer, Serine protease
Distribution

Cytotoxic T cells and natural killer cells (NK); low on granulocytes, B cells, and activated dendritic cells (DCs).

Function
Induction of cell death and apoptosis.
Cell Type
T cells, NK cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Susanto O, et al. 2013. Cell Death Differ. 20:1183.
2. Ebnet K, et al. 1995. EMBO J. 14:4230.
3. Jenne DE, et al. 1988. Immunol. Rev. 103:53.

Gene ID
14938 View all products for this Gene ID
UniProt
View information about Granzyme A on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02.24.2015

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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