PE anti-mouse CD49d Antibody

Pricing & Availability
Clone
R1-2 (See other available formats)
Other Names
α4 integrin, VLA-4 α chain, integrin α4, ITGA4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
R1-2
C57BL/6 splenocytes stained with R1-2 PE
  • R1-2
    C57BL/6 splenocytes stained with R1-2 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
103607 50 µg 90 CHF
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103608 200 µg 250 CHF
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Description

CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
AKR/Cum mouse spontaneous T lymphoma line TK1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The R1-2 antibody has been shown to partially block CD49d mediated interactions and, in combination with the 9C10 (MFR4.B) antibody, completely blocks VCAM-1 binding to VLA-4. Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and T cell costimulation1-4, immunoprecipitation1, and immunohistochemistry of frozen sections5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103629 and 103630).

Application References

(PubMed link indicates BioLegend citation)
  1. Holzmann B, et al. 1989. Cell 56:37. (IP, Block)
  2. Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block)
  3. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
  4. Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block)
  5. Jaspers M, et al. 1995. Differentiation 59:79. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
Product Citations
  1. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  2. Gaylo‐Moynihan A et al. 2019. Immunity. 51(2):298-309 . PubMed
  3. Riedel R, et al. 2020. Nat Commun. 11:2570. PubMed
  4. Pietronigro E, et al. 2019. Sci Rep. 9:12055. PubMed
  5. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  6. Toda S, et al. 2014. Blood. 123:3963. PubMed
  7. Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
RRID
AB_313038 (BioLegend Cat. No. 103607)
AB_313039 (BioLegend Cat. No. 103608)

Antigen Details

Structure
Integrin family, associates with integrin β1 or β7, 150 kD
Distribution

T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells

Function
T cell costimulation, cell-cell and cell-matrix adhesion
Ligand/Receptor
VCAM-1, MAdCAM-1, fibronectin
Cell Type
B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994 J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.

Gene ID
16401 View all products for this Gene ID
UniProt
View information about CD49d on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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