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PE anti-mouse CD366 (Tim-3) Antibody

Pricing & Availability
Clone
RMT3-23 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing 3 protein, hepatitis virus cellular receptor 2, CD366
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMT3-23_PE_CD366_Antibody_1_032224
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) PerCP/Fire™ 780 and anti-mouse CD366 (Tim-3) (clone RMT3-23) PE (left) or rat IgG2a, κ PE isotype control (right).
  • RMT3-23_PE_CD366_Antibody_1_032224
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) PerCP/Fire™ 780 and anti-mouse CD366 (Tim-3) (clone RMT3-23) PE (left) or rat IgG2a, κ PE isotype control (right).
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119703 50 µg 134 CHF
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119704 200 µg 408 CHF
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 119731-119736).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
  2. Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
Product Citations
  1. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  2. Palacios LM, et al. 2022. Biology (Basel). 11:. PubMed
  3. Khalsa JK, et al. 2020. Nat Commun. 3.175. PubMed
  4. Thomas Ciucci et al. 2019. Immunity. 50(1):91-105 . PubMed
  5. Magen A, et al. 2019. Cell Rep. 29:3019. PubMed
  6. Gu M, et al. 2021. Nat Immunol. 22:193. PubMed
  7. Tian H, et al. 2022. Cancer Sci. 113:875. PubMed
  8. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  9. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  10. DeLong JH, et al. 2019. Immunohorizons. 3:13. PubMed
  11. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  12. Chin AL, et al. 2021. Nat Commun. 12:5138. PubMed
  13. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  14. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  15. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  16. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  17. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  18. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  19. Sandu I, et al. 2020. Cell Reports. 32(8):108078. PubMed
  20. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  21. Benci JL et al. 2016. Cell. 167(6):1540-1554 . PubMed
  22. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  23. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  24. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  25. Schofield L, et al. 2017. BMC Med. 10.1186/s12916-017-0883-8. PubMed
  26. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
RRID
AB_345377 (BioLegend Cat. No. 119703)
AB_345378 (BioLegend Cat. No. 119704)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Expressed on activated Th1 lymphocytes and CD11b+ macrophages

Function
May play a role in the development of immune responses and the development of Th1-mediated responses
Ligand/Receptor
Putative ligand on resting CD4+ lymphocytes
Cell Type
Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.

Gene ID
171285 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 04.22.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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