Loading...

PE anti-mouse CD140a Antibody

Pricing & Availability
Clone
APA5 (See other available formats)
Regulatory Status
RUO
Other Names
PDGF receptor-α, PDGFR-α
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
APA5_PE_CD140a_Antibody_071217
Mouse fibroblast NIH/3T3 cells were stained with CD140a (clone APA5) PE (filled histogram) or Rat IgG2a, κ PE isotype control (open histogram).
  • APA5_PE_CD140a_Antibody_071217
    Mouse fibroblast NIH/3T3 cells were stained with CD140a (clone APA5) PE (filled histogram) or Rat IgG2a, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
135905 50 µg 173 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
135906 200 µg 426 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PDGFR-α-hIgG1 recombinant fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Takakura N, et al. 1996. J. Invest. Dermatol. 107:770.
  2. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block)
  3. Chen H, et al. 2015. ASN Neuro 8:7. PubMed.
  4. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)
Product Citations
  1. Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed
  2. Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
  3. Zelic M, et al. 2021. Cell Reports. 35(6):109112. PubMed
  4. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  5. Buechler MB, et al. 2021. Nature. 593:575. PubMed
  6. Wagner G, et al. 2017. Sci Rep. 7:40881. PubMed
  7. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  8. Salzer MC et al. 2018. Cell. 175(6):1575-1590 . PubMed
  9. Chen M, et al. 2020. Sci Adv. 6:eaax9605. PubMed
  10. Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
  11. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  12. Cardot-Ruffino V, et al. 2020. Genesis. 58:e23359. PubMed
  13. Lu A, et al. 2020. Sci Rep. 10:7075. PubMed
  14. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  15. Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
  16. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  17. Diekmann U, et al. 2019. Sci Rep. 9:996. PubMed
  18. Frondelli MJ, et al. 2021. Neurotrauma Rep. 2:285. PubMed
  19. Ito K, et al. 2014. J Biol Chem. 289:29892. PubMed
  20. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  21. Cardot-Ruffino V, et al. 2020. Sci Rep. 10:3880. PubMed
  22. Runyan CE, et al. 2020. Aging Cell. 19:00. PubMed
  23. Xi W, et al. 2021. Stem Cell Res Ther. 167:12. PubMed
  24. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  25. Frech S, et al. 2021. J Invest Dermatol. . PubMed
  26. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  27. Nawaz A, et al. 2018. Sci Rep. 6:37. PubMed
  28. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  29. Fung CW, et al. 2022. Cell Discov. 8:61. PubMed
  30. Haider N, et al. 2020. Cell Commun Signal. 18:26. PubMed
  31. Shibamoto M, et al. 2019. Int Heart J. 60:944. PubMed
  32. Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed
  33. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  34. Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed
  35. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  36. Konishi S, et al. 2022. STAR Protoc. 3:101447. PubMed
  37. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  38. Liu J, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed
  39. Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed
  40. Chen Y, et al. 2021. Cancer Cell. 39(4):548-565.e6. PubMed
  41. Kwon H, Kim S 2016. Cell Death Dis. 7:22285. PubMed
  42. Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed
  43. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  44. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  45. Wang Y, et al. 2020. Cell Reports. 31(5):107600. PubMed
  46. Velletri T, et al. 2020. Cell Death Differ. . PubMed
  47. Bravo JI, et al. 2021. STAR Protocols. 2(2):100406. PubMed
  48. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  49. Ferrari N, et al. 2019. Nat Commun. 10:130. PubMed
  50. Velloso FJ, et al. 2022. STAR Protoc. 3:101065. PubMed
RRID
AB_1953268 (BioLegend Cat. No. 135905)
AB_1953269 (BioLegend Cat. No. 135906)

Antigen Details

Structure
Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution

Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.

Function
Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand/Receptor
PDGFs
Cell Type
Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551
2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124
3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883

Gene ID
18595 View all products for this Gene ID
UniProt
View information about CD140a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account